Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3526152..3526770 Replicon chromosome
Accession NZ_CP085619
Organism Escherichia coli strain EC32

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LJN50_RS17390 Protein ID WP_001291435.1
Coordinates 3526552..3526770 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LJN50_RS17385 Protein ID WP_000344800.1
Coordinates 3526152..3526526 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJN50_RS17375 (3521241) 3521241..3522434 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LJN50_RS17380 (3522457) 3522457..3525606 + 3150 WP_001132478.1 efflux RND transporter permease AcrB -
LJN50_RS17385 (3526152) 3526152..3526526 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LJN50_RS17390 (3526552) 3526552..3526770 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LJN50_RS17395 (3526943) 3526943..3527494 + 552 WP_000102539.1 maltose O-acetyltransferase -
LJN50_RS17400 (3527610) 3527610..3528080 + 471 WP_000136192.1 YlaC family protein -
LJN50_RS17405 (3528244) 3528244..3529794 + 1551 WP_001545832.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LJN50_RS17410 (3529836) 3529836..3530189 - 354 WP_000878147.1 DUF1428 family protein -
LJN50_RS17420 (3530568) 3530568..3530879 + 312 WP_000409908.1 MGMT family protein -
LJN50_RS17425 (3530910) 3530910..3531482 - 573 WP_000779841.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T220925 WP_001291435.1 NZ_CP085619:3526552-3526770 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T220925 NZ_CP117305:c2325305-2325021 [Salmonella enterica subsp. enterica serovar Derby]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT220925 WP_000344800.1 NZ_CP085619:3526152-3526526 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT220925 NZ_CP117305:c2325543-2325295 [Salmonella enterica subsp. enterica serovar Derby]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References