Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2468144..2469732 Replicon chromosome
Accession NZ_CP085619
Organism Escherichia coli strain EC32

Toxin (Protein)


Gene name hipA Uniprot ID A0A061YM43
Locus tag LJN50_RS12185 Protein ID WP_001125411.1
Coordinates 2468410..2469732 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A061YNW4
Locus tag LJN50_RS12180 Protein ID WP_001296076.1
Coordinates 2468144..2468410 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJN50_RS12165 (2464512) 2464512..2465963 + 1452 WP_000854638.1 tagaturonate reductase -
LJN50_RS12170 (2466160) 2466160..2467074 + 915 WP_001296077.1 bestrophin family protein -
LJN50_RS12175 (2467078) 2467078..2467836 - 759 WP_001286537.1 trans-aconitate 2-methyltransferase -
LJN50_RS12180 (2468144) 2468144..2468410 + 267 WP_001296076.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
LJN50_RS12185 (2468410) 2468410..2469732 + 1323 WP_001125411.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
LJN50_RS12190 (2470642) 2470642..2471205 + 564 WP_001296074.1 type 1 fimbrial major subunit FimA -
LJN50_RS12195 (2471567) 2471567..2472277 + 711 WP_001195177.1 fimbria/pilus periplasmic chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(7-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49329.96 Da        Isoelectric Point: 7.7906

>T220923 WP_001125411.1 NZ_CP085619:2468410-2469732 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHDRLSREYGIK

Download         Length: 1323 bp

>T220923 NZ_CP117305:816361-816774 [Salmonella enterica subsp. enterica serovar Derby]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGCCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAAAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9987.34 Da        Isoelectric Point: 4.9632

>AT220923 WP_001296076.1 NZ_CP085619:2468144-2468410 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Download         Length: 267 bp

>AT220923 NZ_CP117305:816114-816380 [Salmonella enterica subsp. enterica serovar Derby]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061YM43

Antitoxin

Source ID Structure
AlphaFold DB A0A061YNW4

References