Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1929998..1930829 Replicon chromosome
Accession NZ_CP085619
Organism Escherichia coli strain EC32

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag LJN50_RS09345 Protein ID WP_000854814.1
Coordinates 1929998..1930372 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A641DSP2
Locus tag LJN50_RS09350 Protein ID WP_001546021.1
Coordinates 1930461..1930829 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJN50_RS09310 (1925993) 1925993..1926322 + 330 WP_000450409.1 DUF496 family protein -
LJN50_RS09315 (1926423) 1926423..1926746 - 324 WP_223216378.1 EutP/PduV family microcompartment system protein -
LJN50_RS09320 (1926725) 1926725..1926805 + 81 WP_023441679.1 hypothetical protein -
LJN50_RS09325 (1927016) 1927016..1928557 + 1542 WP_001298859.1 IS21-like element ISEc12 family transposase -
LJN50_RS09330 (1928572) 1928572..1929318 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
LJN50_RS09335 (1929681) 1929681..1929761 - 81 Protein_1827 hypothetical protein -
LJN50_RS09340 (1929807) 1929807..1930001 - 195 WP_000988601.1 DUF5983 family protein -
LJN50_RS09345 (1929998) 1929998..1930372 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
LJN50_RS09350 (1930461) 1930461..1930829 - 369 WP_001546021.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
LJN50_RS09355 (1930909) 1930909..1931130 - 222 WP_000692323.1 DUF987 domain-containing protein -
LJN50_RS09360 (1931193) 1931193..1931669 - 477 WP_217823699.1 RadC family protein -
LJN50_RS09365 (1931685) 1931685..1932158 - 474 WP_001385393.1 antirestriction protein -
LJN50_RS09370 (1932421) 1932421..1933242 - 822 WP_001234710.1 DUF932 domain-containing protein -
LJN50_RS09375 (1933463) 1933463..1933873 - 411 WP_000846704.1 hypothetical protein -
LJN50_RS09380 (1933889) 1933889..1934566 - 678 WP_001362823.1 hypothetical protein -
LJN50_RS09385 (1934702) 1934702..1935772 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T220914 WP_000854814.1 NZ_CP085619:c1930372-1929998 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T220914 NZ_CP117301:c1296540-1296322 [Salmonella enterica subsp. enterica serovar Derby]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAACTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13607.47 Da        Isoelectric Point: 6.3139

>AT220914 WP_001546021.1 NZ_CP085619:c1930829-1930461 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRASIRGLFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT220914 NZ_CP117301:c1296942-1296568 [Salmonella enterica subsp. enterica serovar Derby]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGGTTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A641DSP2

References