Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 965299..965953 Replicon chromosome
Accession NZ_CP085619
Organism Escherichia coli strain EC32

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag LJN50_RS04875 Protein ID WP_000244765.1
Coordinates 965546..965953 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag LJN50_RS04870 Protein ID WP_000354050.1
Coordinates 965299..965565 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJN50_RS04850 (961387) 961387..962820 - 1434 WP_001296350.1 6-phospho-beta-glucosidase BglA -
LJN50_RS04855 (962865) 962865..963176 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
LJN50_RS04860 (963340) 963340..963999 + 660 WP_000250275.1 hemolysin III family protein -
LJN50_RS04865 (964076) 964076..965056 - 981 WP_000886080.1 tRNA-modifying protein YgfZ -
LJN50_RS04870 (965299) 965299..965565 + 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
LJN50_RS04875 (965546) 965546..965953 + 408 WP_000244765.1 protein YgfX Toxin
LJN50_RS04880 (965993) 965993..966514 - 522 WP_001055874.1 flavodoxin FldB -
LJN50_RS04885 (966626) 966626..967522 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LJN50_RS04890 (967547) 967547..968257 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LJN50_RS04895 (968263) 968263..969996 + 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T220912 WP_000244765.1 NZ_CP085619:965546-965953 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T220912 NZ_CP117301:816366-816779 [Salmonella enterica subsp. enterica serovar Derby]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGCCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAAAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT220912 WP_000354050.1 NZ_CP085619:965299-965565 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT220912 NZ_CP117301:816119-816385 [Salmonella enterica subsp. enterica serovar Derby]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References