Detailed information of TA system TA022091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 846758..847461
• Replicon: chromosome
• Accession: NC_009483
• Organism: Geobacter uraniireducens Rf4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A5GBX6
• Locus tag: GURA_RS03635
• Protein ID: WP_011937647.1
• Coordinates: 846758..847183 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A5GBX7
• Locus tag: GURA_RS03640
• Protein ID: WP_011937648.1
• Coordinates: 847180..847461 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GURA_RS03615	843368..843781	+	414	WP_011937643.1	hypothetical protein	-
GURA_RS03620	843813..844487	+	675	WP_011937644.1	NAD(P)/FAD-dependent oxidoreductase	-
GURA_RS03625	844575..845333	+	759	WP_011937645.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
GURA_RS03630	845685..846662	+	978	WP_011937646.1	4Fe-4S binding protein	-
GURA_RS03635	846758..847183	-	426	WP_011937647.1	type II toxin-antitoxin system VapC family toxin	Toxin
GURA_RS03640	847180..847461	-	282	WP_011937648.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GURA_RS03645	847700..848119	-	420	WP_011937649.1	TraR/DksA family transcriptional regulator	-
GURA_RS03650	848231..849565	-	1335	WP_041245717.1	30S ribosomal protein S12 methylthiotransferase RimO	-
GURA_RS03655	849691..850176	-	486	WP_011937651.1	YajQ family cyclic di-GMP-binding protein	-
GURA_RS03660	850223..850948	-	726	WP_011937652.1	outer membrane lipoprotein carrier protein LolA	-
GURA_RS03665	851041..851874	-	834	WP_011937653.1	outer membrane lipoprotein carrier protein LolA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (12-76)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15573.83 Da
• Isoelectric Point: 4.6940

>T22091 WP_011937647.1 NC_009483:c847183-846758 [Geobacter uraniireducens Rf4]
VKFLLDTCLISELVKKEPNAAVVNWLDEQDEQGLFLSVLTLGELQKGISKLSGSTRKDELQAWVEHDLAERFAGRILDLD
LETALAWGTLQGDAERKGEKLPVMDSLIAATATAHGLIVVTRNVKDIERCRAKVYSPWGDA

Nucleotide

• Length: 426 bp

>T22091 NC_009483:c847183-846758 [Geobacter uraniireducens Rf4]
GTGAAATTTCTTCTCGACACCTGCCTGATCTCCGAGCTGGTGAAAAAGGAACCGAATGCCGCTGTGGTAAACTGGCTGGA
CGAACAGGATGAGCAGGGTCTTTTCCTGAGTGTCCTGACCCTTGGAGAACTGCAAAAGGGAATCAGCAAGCTTTCCGGCA
GCACCAGGAAAGATGAGCTTCAGGCGTGGGTCGAGCATGATCTTGCGGAGCGTTTCGCCGGACGTATCCTTGATCTCGAC
CTGGAAACGGCTCTGGCCTGGGGAACGCTTCAGGGGGATGCTGAACGGAAGGGGGAAAAGCTGCCGGTAATGGATTCACT
CATAGCTGCCACGGCCACGGCACACGGATTGATCGTGGTAACGCGCAATGTCAAGGATATCGAGCGCTGCAGGGCCAAGG
TTTACAGTCCGTGGGGCGATGCATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10760.15 Da
• Isoelectric Point: 8.7734

>AT22091 WP_011937648.1 NC_009483:c847461-847180 [Geobacter uraniireducens Rf4]
MKANKKDRLPHEREWQLQEAKNRLSQVVDSALHDGPQTITLRGKPAAVLVSFEEFKKLTQPRSTLSQFFRQSPLHDTELD
LSRSADFSREVEL

Nucleotide

• Length: 282 bp

>AT22091 NC_009483:c847461-847180 [Geobacter uraniireducens Rf4]
ATGAAAGCCAATAAAAAAGACCGATTGCCCCACGAACGTGAATGGCAGCTTCAGGAAGCCAAGAACCGATTGAGCCAGGT
AGTGGACTCGGCTCTTCATGATGGTCCGCAAACCATCACCTTGCGGGGCAAACCGGCGGCGGTCTTAGTCTCTTTTGAGG
AATTCAAAAAGCTGACTCAACCGCGGTCCACGTTATCGCAGTTCTTCAGACAATCACCGCTTCATGATACAGAGCTCGAT
CTCAGCCGCAGTGCCGATTTCTCCCGTGAGGTGGAACTGTGA

Structures

Toxin

• AlphaFold DB: A5GBX6

Antitoxin

• AlphaFold DB: A5GBX7