Detailed information of TA system TA220903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3771823..3772508
• Replicon: chromosome
• Accession: NZ_CP085532
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TIR9
• Locus tag: LJF87_RS17720
• Protein ID: WP_003417998.1
• Coordinates: 3772098..3772508 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LJF87_RS17715
• Protein ID: WP_003912220.1
• Coordinates: 3771823..3772101 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF87_RS17685 (LJF87_17670)	3767248..3768156	-	909	WP_003417954.1	presqualene diphosphate synthase HpnD	-
LJF87_RS17690 (LJF87_17675)	3768185..3769264	-	1080	WP_003417957.1	(2E,6E)-farnesyl diphosphate synthase	-
LJF87_RS17695 (LJF87_17680)	3769413..3770333	+	921	WP_003912218.1	class I SAM-dependent methyltransferase	-
LJF87_RS17700 (LJF87_17685)	3770397..3770522	+	126	Protein_3496	hypothetical protein	-
LJF87_RS17705 (LJF87_17690)	3770525..3770818	-	294	Protein_3497	helix-turn-helix domain containing protein	-
LJF87_RS17710 (LJF87_17695)	3770880..3771767	+	888	WP_003900050.1	alpha-ketoglutarate-dependent sulfate ester dioxygenase	-
LJF87_RS17715 (LJF87_17700)	3771823..3772101	+	279	WP_003912220.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LJF87_RS17720 (LJF87_17705)	3772098..3772508	+	411	WP_003417998.1	type II toxin-antitoxin system VapC family toxin	Toxin
LJF87_RS17725 (LJF87_17710)	3772541..3774277	-	1737	WP_003418002.1	cholesterol oxidase	-
LJF87_RS17730 (LJF87_17715)	3774333..3775460	-	1128	WP_003418005.1	GuaB3 family IMP dehydrogenase-related protein	-
LJF87_RS17735 (LJF87_17720)	3775480..3777069	-	1590	WP_003900682.1	IMP dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14695.84 Da
• Isoelectric Point: 5.1784

>T220903 WP_003417998.1 NZ_CP085532:3772098-3772508 [Mycobacterium tuberculosis variant bovis BCG]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVELMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T220903 NZ_CP117300:c150693-150412 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10280.75 Da
• Isoelectric Point: 10.1819

>AT220903 WP_003912220.1 NZ_CP085532:3771823-3772101 [Mycobacterium tuberculosis variant bovis BCG]
VEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEPARGRKRT
LSDVLNEMRDEQ

Nucleotide

• Length: 279 bp

>AT220903 NZ_CP117300:c150917-150690 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: G0TIR9