Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3046834..3047404 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag LJF87_RS14455 Protein ID WP_003414166.1
Coordinates 3046834..3047190 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag LJF87_RS14460 Protein ID WP_003901465.1
Coordinates 3047174..3047404 (-) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS14435 (LJF87_14430) 3042286..3043974 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
LJF87_RS14440 (LJF87_14435) 3043978..3044304 - 327 WP_003414157.1 hypothetical protein -
LJF87_RS14445 (LJF87_14440) 3044477..3045064 + 588 WP_003914429.1 DUF3558 family protein -
LJF87_RS14450 (LJF87_14445) 3045083..3046732 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
LJF87_RS14455 (LJF87_14450) 3046834..3047190 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
LJF87_RS14460 (LJF87_14455) 3047174..3047404 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
LJF87_RS14465 (LJF87_14460) 3047447..3048490 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
LJF87_RS14470 (LJF87_14465) 3048489..3048956 + 468 WP_003414177.1 DUF1778 domain-containing protein -
LJF87_RS14475 (LJF87_14470) 3049132..3049386 - 255 WP_003917684.1 hypothetical protein -
LJF87_RS14480 (LJF87_14475) 3049534..3049938 + 405 WP_003414181.1 hypothetical protein -
LJF87_RS14485 (LJF87_14480) 3049935..3050126 + 192 WP_003414184.1 hypothetical protein -
LJF87_RS14490 (LJF87_14485) 3050343..3050603 + 261 Protein_2859 transposase -
LJF87_RS14495 (LJF87_14490) 3051713..3051970 + 258 WP_003899489.1 hypothetical protein -
LJF87_RS14500 (LJF87_14495) 3052075..3052386 + 312 WP_010950777.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-113)

Antitoxin

(3-29)


Sequences


Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T220894 WP_003414166.1 NZ_CP085532:c3047190-3046834 [Mycobacterium tuberculosis variant bovis BCG]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T220894 NZ_CP117299:c1624001-1623783 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT220894 WP_003901465.1 NZ_CP085532:c3047404-3047174 [Mycobacterium tuberculosis variant bovis BCG]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT220894 NZ_CP117299:c1624403-1624029 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS


Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References