Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2816277..2816908 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag LJF87_RS13200 Protein ID WP_003413174.1
Coordinates 2816531..2816908 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag LJF87_RS13195 Protein ID WP_003413167.1
Coordinates 2816277..2816534 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS13160 (LJF87_13150) 2811535..2811849 + 315 WP_009937839.1 hypothetical protein -
LJF87_RS13165 (LJF87_13155) 2812145..2813356 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
LJF87_RS13170 (LJF87_13160) 2813483..2814142 + 660 WP_003900846.1 LppA family lipoprotein -
LJF87_RS13175 (LJF87_13165) 2814139..2814798 + 660 WP_003900847.1 LppA family lipoprotein -
LJF87_RS13180 (LJF87_13170) 2814795..2815457 + 663 WP_003900848.1 LppA family lipoprotein -
LJF87_RS13185 (LJF87_13175) 2815454..2815732 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
LJF87_RS13190 (LJF87_13180) 2815825..2816238 + 414 WP_003413164.1 PIN domain nuclease -
LJF87_RS13195 (LJF87_13185) 2816277..2816534 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
LJF87_RS13200 (LJF87_13190) 2816531..2816908 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
LJF87_RS13205 (LJF87_13195) 2816924..2817298 - 375 WP_003413177.1 hypothetical protein -
LJF87_RS13210 (LJF87_13200) 2817398..2817793 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
LJF87_RS13215 (LJF87_13205) 2817790..2818035 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
LJF87_RS13220 (LJF87_13210) 2818446..2818865 - 420 WP_003413190.1 A24 family peptidase -
LJF87_RS13225 (LJF87_13215) 2818877..2819685 - 809 Protein_2610 shikimate dehydrogenase -
LJF87_RS13230 (LJF87_13220) 2819682..2820935 - 1254 WP_274485783.1 endolytic transglycosylase MltG -
LJF87_RS13235 (LJF87_13225) 2820928..2821440 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T220889 WP_003413174.1 NZ_CP085532:2816531-2816908 [Mycobacterium tuberculosis variant bovis BCG]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T220889 NZ_CP117299:879574-879882 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGGTTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT220889 WP_003413167.1 NZ_CP085532:2816277-2816534 [Mycobacterium tuberculosis variant bovis BCG]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT220889 NZ_CP117299:879332-879571 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGTCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCTGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGCGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References