Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2755098..2755750 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name vapC Uniprot ID G0TPX1
Locus tag LJF87_RS12905 Protein ID WP_003412752.1
Coordinates 2755325..2755750 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag LJF87_RS12900 Protein ID WP_003412749.1
Coordinates 2755098..2755319 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS12885 (LJF87_12875) 2753338..2753544 - 207 WP_003899347.1 hypothetical protein -
LJF87_RS12890 (LJF87_12880) 2753680..2754303 + 624 WP_003900858.1 TIGR00725 family protein -
LJF87_RS12895 (LJF87_12885) 2754293..2755045 + 753 WP_003901416.1 hypothetical protein -
LJF87_RS12900 (LJF87_12890) 2755098..2755319 + 222 WP_003412749.1 antitoxin Antitoxin
LJF87_RS12905 (LJF87_12895) 2755325..2755750 + 426 WP_003412752.1 type II toxin-antitoxin system VapC family toxin Toxin
LJF87_RS12910 (LJF87_12900) 2755773..2756954 - 1182 WP_010950729.1 dihydrolipoamide acetyltransferase family protein -
LJF87_RS12915 (LJF87_12905) 2756951..2757997 - 1047 WP_010950730.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
LJF87_RS12920 (LJF87_12910) 2758008..2759111 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
LJF87_RS12925 (LJF87_12915) 2759370..2760191 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
LJF87_RS12930 (LJF87_12920) 2760188..2760745 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15392.89 Da        Isoelectric Point: 9.2386

>T220886 WP_003412752.1 NZ_CP085532:2755325-2755750 [Mycobacterium tuberculosis variant bovis BCG]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRVSTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T220886 NZ_CP117299:351800-352111 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGTTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCCTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT220886 WP_003412749.1 NZ_CP085532:2755098-2755319 [Mycobacterium tuberculosis variant bovis BCG]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT220886 NZ_CP117299:352112-352402 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References