Detailed information of TA system TA220881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2220125..2220751
• Replicon: chromosome
• Accession: NZ_CP085532
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: LJF87_RS10380
• Protein ID: WP_003410075.1
• Coordinates: 2220353..2220751 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LJF87_RS10375
• Protein ID: WP_019283586.1
• Coordinates: 2220125..2220352 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF87_RS10365 (LJF87_10360)	2218164..2218508	-	345	WP_003410065.1	ferredoxin family protein	-
LJF87_RS10370 (LJF87_10365)	2218697..2219866	-	1170	WP_003899126.1	ATP-binding protein	-
LJF87_RS10375 (LJF87_10370)	2220125..2220352	+	228	WP_019283586.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LJF87_RS10380 (LJF87_10375)	2220353..2220751	+	399	WP_003410075.1	PIN domain nuclease	Toxin
LJF87_RS10385 (LJF87_10380)	2220934..2221365	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
LJF87_RS10390 (LJF87_10385)	2221466..2221900	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
LJF87_RS10395 (LJF87_10390)	2222337..2222516	-	180	Protein_2054	hypothetical protein	-
LJF87_RS10400 (LJF87_10395)	2222604..2223221	+	618	WP_011799237.1	IS110 family transposase	-
LJF87_RS10405 (LJF87_10400)	2223175..2223765	+	591	WP_003899131.1	IS110 family transposase	-
LJF87_RS10410 (LJF87_10405)	2223893..2225149	-	1257	WP_003410095.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T220881 WP_003410075.1 NZ_CP085532:2220353-2220751 [Mycobacterium tuberculosis variant bovis BCG]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T220881 NZ_CP117298:c3656530-3656102 [Mycobacterium tuberculosis variant bovis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8347.14 Da
• Isoelectric Point: 4.0995

>AT220881 WP_019283586.1 NZ_CP085532:2220125-2220352 [Mycobacterium tuberculosis variant bovis BCG]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSDDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT220881 NZ_CP117298:c3656776-3656534 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• PDB: 4CHG