Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mbcAT/RES-TIGR02293
Location 2204677..2205575 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name mbcT Uniprot ID P64908
Locus tag LJF87_RS10295 Protein ID WP_003410001.1
Coordinates 2204677..2205237 (-) Length 187 a.a.

Antitoxin (Protein)


Gene name mbcA Uniprot ID P64910
Locus tag LJF87_RS10300 Protein ID WP_003410003.1
Coordinates 2205234..2205575 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS10265 (LJF87_10260) 2199846..2200499 - 654 WP_003409976.1 cutinase Cfp21 -
LJF87_RS10270 (LJF87_10265) 2200614..2200844 + 231 WP_003409980.1 DUF167 domain-containing protein -
LJF87_RS10275 (LJF87_10270) 2200929..2201840 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
LJF87_RS10280 (LJF87_10275) 2201949..2202548 + 600 WP_003409989.1 L-lysine exporter -
LJF87_RS10285 (LJF87_10280) 2202964..2203392 + 429 WP_003409992.1 cellulose-binding protein -
LJF87_RS10290 (LJF87_10285) 2203618..2204157 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
LJF87_RS10295 (LJF87_10290) 2204677..2205237 - 561 WP_003410001.1 RES family NAD+ phosphorylase Toxin
LJF87_RS10300 (LJF87_10295) 2205234..2205575 - 342 WP_003410003.1 DUF2384 domain-containing protein Antitoxin
LJF87_RS10305 (LJF87_10300) 2205661..2205918 + 258 WP_003410006.1 hypothetical protein -
LJF87_RS10310 (LJF87_10305) 2205819..2206154 - 336 WP_003410009.1 dehydrogenase -
LJF87_RS10315 (LJF87_10310) 2206243..2206314 - 72 Protein_2038 type II toxin-antitoxin system PemK/MazF family toxin -
LJF87_RS10320 (LJF87_10315) 2206314..2207218 - 905 Protein_2039 APC family permease -
LJF87_RS10325 (LJF87_10320) 2207289..2208902 + 1614 WP_003410042.1 NAD(P)/FAD-dependent oxidoreductase -
LJF87_RS10330 (LJF87_10325) 2208912..2209664 + 753 WP_010950640.1 acyl-[acyl-carrier-protein] thioesterase -
LJF87_RS10335 (LJF87_10330) 2209740..2210522 + 783 WP_003410047.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(25-148)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(69-110)


Sequences

Toxin        


Download         Length: 187 a.a.        Molecular weight: 20248.70 Da        Isoelectric Point: 4.5091

>T220880 WP_003410001.1 NZ_CP085532:c2205237-2204677 [Mycobacterium tuberculosis variant bovis BCG]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Download         Length: 561 bp

>T220880 NZ_CP085532:c2205237-2204677 [Mycobacterium tuberculosis variant bovis BCG]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12488.33 Da        Isoelectric Point: 4.8359

>AT220880 WP_003410003.1 NZ_CP085532:c2205575-2205234 [Mycobacterium tuberculosis variant bovis BCG]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Download         Length: 342 bp

>AT220880 NZ_CP085532:c2205575-2205234 [Mycobacterium tuberculosis variant bovis BCG]
ATGGGAGTAAATGTGCTCGCCTCGACCGTGTCGGGTGCGATCGAGCGCTTGGGATTGACCTACGAGGAAGTCGGTGACAT
CGTCGATGCCTCGCCGCGTTCCGTGGCGCGATGGACCGCAGGTCAGGTGGTTCCCCAACGCCTCAACAAGCAACGACTTA
TCGAGCTGGCCTATGTCGCCGACGCCCTCGCGGAAGTGCTGCCGCGTGACCAGGCGAACGTGTGGATGTTTTCGCCGAAT
CGGTTACTGGAACACCGCAAGCCTGCCGACCTCGTGCGAGACGGCGAGTACCAACGCGTGTTGGCGCTCATCGACGCGAT
GGCGGAGGGAGTGTTCGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FKG

Antitoxin

Source ID Structure
PDB 6FKG
AlphaFold DB A0A7U4FAE4

References