Detailed information of TA system TA022088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 16045..16634
• Replicon: plasmid pACRY05
• Accession: NC_009471
• Organism: Acidiphilium cryptum JF-5

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A5FUB2
• Locus tag: ACRY_RS02270
• Protein ID: WP_011930859.1
• Coordinates: 16045..16371 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A5FUB3
• Locus tag: ACRY_RS02275
• Protein ID: WP_011930860.1
• Coordinates: 16371..16634 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACRY_RS02245	11355..12896	+	1542	WP_011930854.1	IS21 family transposase	-
ACRY_RS02250	12908..13660	+	753	WP_011930855.1	IS21-like element helper ATPase IstB	-
ACRY_RS02255	13814..14719	+	906	Protein_9	IS110 family transposase	-
ACRY_RS19545	14965..15755	-	791	Protein_10	IS630 family transposase	-
ACRY_RS02270	16045..16371	-	327	WP_011930859.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ACRY_RS02275	16371..16634	-	264	WP_011930860.1	antitoxin	Antitoxin
ACRY_RS02280	16863..17522	+	660	WP_011930861.1	AAA family ATPase	-
ACRY_RS02285	17537..17812	+	276	WP_011930862.1	CopG family transcriptional regulator	-
ACRY_RS19550	17799..18035	+	237	WP_196796265.1	hypothetical protein	-
ACRY_RS02290	18125..19235	+	1111	Protein_16	IS110 family transposase	-
ACRY_RS02295	19560..19934	+	375	Protein_17	type IV secretory system conjugative DNA transfer family protein	-
ACRY_RS18780	19952..20047	-	96	Protein_18	DUF4113 domain-containing protein	-
ACRY_RS19555	20111..20242	+	132	Protein_19	IS256 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37155	37155
	-	inside	IScluster/Tn	-	-	13787..19235	5448

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11634.36 Da
• Isoelectric Point: 7.1341

>T22088 WP_011930859.1 NC_009471:c16371-16045 [Acidiphilium cryptum JF-5]
MNRGDIYLVDLEPTAGHEQRGHRPVLVVSPDAFNRLTQCPVILPITNGGDFARRVGFAVPISGIKTTGVVRCDQPRALDL
SARNARKVDTLPAAILDDVLATAATLFE

Nucleotide

• Length: 327 bp

>T22088 NC_009471:c16371-16045 [Acidiphilium cryptum JF-5]
ATGAATCGCGGCGATATCTATCTAGTCGATCTTGAGCCGACAGCAGGACACGAGCAGCGCGGCCATCGGCCGGTGCTGGT
CGTGTCCCCGGATGCCTTCAACCGCCTGACACAATGCCCGGTCATCCTGCCGATAACCAACGGCGGCGATTTCGCCAGGC
GTGTCGGTTTCGCGGTTCCAATCAGCGGCATCAAAACAACGGGCGTCGTGCGGTGCGATCAACCGCGCGCGCTCGATCTG
AGCGCCCGCAATGCTCGCAAGGTAGACACGCTGCCGGCGGCGATCCTGGATGACGTACTAGCCACGGCCGCCACGCTGTT
CGAGTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9291.84 Da
• Isoelectric Point: 5.0210

>AT22088 WP_011930860.1 NC_009471:c16634-16371 [Acidiphilium cryptum JF-5]
MHTTNLRKVGGSIMLAVPPALLDVLHLAPGTKVGLAVDNGRLVVEPQARPRYTMADLLAEAEAAGVYPLPPEEREWIDAP
AVGKELI

Nucleotide

• Length: 264 bp

>AT22088 NC_009471:c16634-16371 [Acidiphilium cryptum JF-5]
ATGCACACCACAAACTTGCGCAAAGTCGGCGGGTCTATCATGCTAGCCGTGCCACCCGCACTGCTCGATGTCCTGCACCT
GGCACCGGGTACCAAGGTCGGCCTGGCCGTCGATAACGGCCGCCTGGTCGTCGAGCCGCAAGCCCGGCCGCGCTACACGA
TGGCCGATCTGCTCGCCGAGGCCGAAGCAGCCGGCGTCTATCCGCTGCCGCCGGAAGAACGCGAATGGATCGACGCCCCG
GCAGTTGGAAAAGAACTGATTTGA

Structures

Toxin

• AlphaFold DB: A5FUB2

Antitoxin

• AlphaFold DB: A5FUB3