Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1925653..1926266 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag LJF87_RS08975 Protein ID WP_003408465.1
Coordinates 1925653..1926042 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag LJF87_RS08980 Protein ID WP_003408469.1
Coordinates 1926039..1926266 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS08940 (LJF87_08920) 1921283..1922197 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
LJF87_RS08945 (LJF87_08925) 1922200..1923030 + 831 WP_003408448.1 cyclase family protein -
LJF87_RS08950 (LJF87_08930) 1923030..1923380 + 351 WP_003898986.1 cupin domain-containing protein -
LJF87_RS08955 (LJF87_08935) 1923433..1924250 + 818 Protein_1767 3-keto-5-aminohexanoate cleavage protein -
LJF87_RS08960 (LJF87_08940) 1924264..1925043 + 780 WP_003408460.1 IclR family transcriptional regulator -
LJF87_RS08970 (LJF87_08950) 1925474..1925605 + 132 Protein_1769 IS3 family transposase -
LJF87_RS08975 (LJF87_08955) 1925653..1926042 - 390 WP_003408465.1 type II toxin-antitoxin system VapC family toxin Toxin
LJF87_RS08980 (LJF87_08960) 1926039..1926266 - 228 WP_003408469.1 antitoxin Antitoxin
LJF87_RS08985 (LJF87_08965) 1926484..1927968 + 1485 WP_010950580.1 biotin carboxylase -
LJF87_RS08990 (LJF87_08970) 1927965..1929212 + 1248 WP_003408476.1 serine hydrolase -
LJF87_RS08995 (LJF87_08975) 1929255..1929674 - 420 WP_003408483.1 hypothetical protein -
LJF87_RS09000 (LJF87_08980) 1929664..1930374 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T220874 WP_003408465.1 NZ_CP085532:c1926042-1925653 [Mycobacterium tuberculosis variant bovis BCG]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T220874 NZ_CP117298:c3020995-3020600 [Mycobacterium tuberculosis variant bovis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT220874 WP_003408469.1 NZ_CP085532:c1926266-1926039 [Mycobacterium tuberculosis variant bovis BCG]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT220874 NZ_CP117298:c3020574-3020308 [Mycobacterium tuberculosis variant bovis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References