Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1572284..1572939 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name vapC Uniprot ID A0A7U4FAR1
Locus tag LJF87_RS07445 Protein ID WP_003407268.1
Coordinates 1572284..1572685 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag LJF87_RS07450 Protein ID WP_003407272.1
Coordinates 1572682..1572939 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS07430 (LJF87_07395) 1568128..1569513 - 1386 WP_003407260.1 cytochrome P450 -
LJF87_RS07435 (LJF87_07400) 1569591..1570625 + 1035 WP_003407264.1 AraC family transcriptional regulator -
LJF87_RS07440 (LJF87_07405) 1570671..1572029 - 1359 WP_010950541.1 PE family protein -
LJF87_RS07445 (LJF87_07410) 1572284..1572685 - 402 WP_003407268.1 type II toxin-antitoxin system VapC family toxin Toxin
LJF87_RS07450 (LJF87_07415) 1572682..1572939 - 258 WP_003407272.1 CopG family transcriptional regulator Antitoxin
LJF87_RS07455 (LJF87_07420) 1573022..1573981 - 960 WP_011799179.1 alpha/beta hydrolase -
LJF87_RS07460 (LJF87_07425) 1574006..1574968 - 963 WP_003407279.1 alpha/beta hydrolase -
LJF87_RS07465 (LJF87_07430) 1574967..1575704 + 738 WP_031647000.1 lysoplasmalogenase -
LJF87_RS07470 (LJF87_07435) 1575785..1577752 + 1968 WP_003407285.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14894.39 Da        Isoelectric Point: 11.6897

>T220871 WP_003407268.1 NZ_CP085532:c1572685-1572284 [Mycobacterium tuberculosis variant bovis BCG]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T220871 NZ_CP117298:c2830827-2830432 [Mycobacterium tuberculosis variant bovis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT220871 WP_003407272.1 NZ_CP085532:c1572939-1572682 [Mycobacterium tuberculosis variant bovis BCG]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT220871 NZ_CP117298:c2831069-2830824 [Mycobacterium tuberculosis variant bovis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FAR1

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References