Detailed information of TA system TA022087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ParST/RES-TIGR02293
• Location: 148857..149917
• Replicon: plasmid pACRY02
• Accession: NC_009468
• Organism: Acidiphilium cryptum JF-5

Toxin (Protein)

• Gene name: ParT
• Uniprot ID: -
• Locus tag: ACRY_RS18610
• Protein ID: WP_081432835.1
• Coordinates: 148857..149345 (-)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: ParS
• Uniprot ID: A5FTV8
• Locus tag: ACRY_RS01540
• Protein ID: WP_011930614.1
• Coordinates: 149342..149917 (-)
• Length: 192 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACRY_RS18600	144550..144882	-	333	Protein_153	hypothetical protein	-
ACRY_RS01525	145475..146626	-	1152	WP_011930617.1	hypothetical protein	-
ACRY_RS19465	147195..147503	+	309	WP_155856316.1	hypothetical protein	-
ACRY_RS01535	147715..148842	+	1128	WP_011930615.1	site-specific integrase	-
ACRY_RS18610	148857..149345	-	489	WP_081432835.1	RES family NAD+ phosphorylase	Toxin
ACRY_RS01540	149342..149917	-	576	WP_011930614.1	DUF2384 domain-containing protein	Antitoxin
ACRY_RS18070	150068..150529	+	462	WP_011930613.1	hypothetical protein	-
ACRY_RS01550	150680..151297	+	618	WP_011930612.1	ATP-binding protein	-
ACRY_RS01555	151956..152618	+	663	WP_011930611.1	ParA family protein	-
ACRY_RS01560	153446..154381	+	936	WP_011930610.1	hypothetical protein	-
ACRY_RS19470	154606..154839	+	234	WP_155856309.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..187422	187422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (5-146)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (139-188)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17990.42 Da
• Isoelectric Point: 6.8734

>T22087 WP_081432835.1 NC_009468:c149345-148857 [Acidiphilium cryptum JF-5]
VTASFWRIGTDTRDYTADDRSGKGAEITGGRWNDVGTPMVYAASSRALACLETVVHLNGSGLPLNRYLVEIIIPDDLVRS
AETYDEASLPVGWDAEPPGKVSIDLGTTWVRDKRSAALFVPSVIVPEELNVLINPTHPGARKIEFHKRRKWLYDPRILAP
RH

Nucleotide

• Length: 489 bp

>T22087 NC_009468:c149345-148857 [Acidiphilium cryptum JF-5]
GTGACCGCGAGTTTCTGGAGGATCGGGACAGACACTCGGGATTATACGGCGGACGACAGGAGCGGAAAGGGGGCGGAGAT
CACGGGCGGACGCTGGAATGACGTTGGCACCCCAATGGTTTACGCAGCATCCTCGCGCGCGCTGGCTTGCCTCGAAACTG
TGGTGCATCTGAACGGGAGCGGTTTGCCGCTGAACCGATATCTGGTCGAGATCATCATCCCGGATGATCTGGTCAGATCG
GCAGAGACATATGATGAGGCATCGCTGCCTGTGGGGTGGGATGCGGAGCCTCCCGGGAAGGTGAGTATCGACTTGGGAAC
CACTTGGGTCCGGGACAAAAGATCGGCGGCATTATTCGTCCCATCCGTTATCGTTCCCGAGGAGCTGAACGTCCTCATCA
ACCCGACCCATCCAGGTGCCCGCAAAATAGAGTTTCACAAGCGCCGGAAATGGCTGTACGACCCGCGCATACTTGCGCCG
CGGCATTGA

Antitoxin

Protein

• Length: 192 a.a.
• Molecular weight: 20520.77 Da
• Isoelectric Point: 10.7310

>AT22087 WP_011930614.1 NC_009468:c149917-149342 [Acidiphilium cryptum JF-5]
MVTTTVSKAATRTPSGPKANKPSHKAPKTSRALKVPMTSPGSPAAKGGTDVSYLEYFYQAPMRQIEMIRRGIPALTVKRI
AGDLRIEQDTLLRALNLSVATLNRKVAQAKNLPADESERIAGIARLVGQVEAMVKESGDPEGFNAAEWLSRWLREPLPAL
GGQMPIKLLDTMAGQALVSQALSRIQSGAYA

Nucleotide

• Length: 576 bp

>AT22087 NC_009468:c149917-149342 [Acidiphilium cryptum JF-5]
ATGGTTACCACGACTGTGAGCAAAGCCGCGACCAGAACACCTTCAGGTCCGAAGGCAAACAAGCCTTCGCACAAGGCCCC
CAAGACATCTCGCGCGCTTAAGGTTCCCATGACATCACCCGGATCCCCGGCGGCAAAAGGTGGGACCGATGTGTCCTACC
TGGAATACTTTTATCAGGCGCCTATGCGCCAAATCGAGATGATCAGGAGGGGGATTCCCGCGCTCACCGTAAAGCGGATC
GCAGGTGATCTCAGGATCGAGCAGGACACTTTGCTCCGTGCGCTCAACCTGAGTGTAGCCACCCTGAATCGAAAGGTCGC
CCAGGCGAAGAATCTGCCGGCCGATGAGAGCGAGCGCATTGCCGGTATTGCGCGACTGGTGGGGCAGGTCGAGGCGATGG
TGAAGGAATCCGGTGATCCAGAGGGATTTAACGCCGCAGAATGGCTCTCGCGCTGGTTGCGCGAGCCATTGCCAGCGCTC
GGCGGTCAGATGCCTATCAAATTGCTTGACACGATGGCGGGGCAAGCCCTCGTCTCACAGGCATTGTCCCGCATTCAGAG
CGGGGCCTATGCGTGA

Structures

Antitoxin

• AlphaFold DB: A5FTV8