Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1233914..1234545 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag LJF87_RS05900 Protein ID WP_003405820.1
Coordinates 1233914..1234225 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag LJF87_RS05905 Protein ID WP_003405836.1
Coordinates 1234225..1234545 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS05880 (LJF87_05845) 1229396..1230820 - 1425 WP_003405805.1 class II fumarate hydratase -
LJF87_RS05885 (LJF87_05850) 1230851..1231939 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
LJF87_RS05890 (LJF87_05855) 1231995..1232639 + 645 WP_174813088.1 DUF4245 domain-containing protein -
LJF87_RS05895 (LJF87_05860) 1232646..1233802 - 1157 Protein_1161 AI-2E family transporter -
LJF87_RS05900 (LJF87_05865) 1233914..1234225 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
LJF87_RS05905 (LJF87_05870) 1234225..1234545 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
LJF87_RS05910 (LJF87_05875) 1234555..1236091 + 1537 Protein_1164 carboxylesterase/lipase family protein -
LJF87_RS05915 (LJF87_05880) 1236098..1237210 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
LJF87_RS05920 (LJF87_05885) 1237220..1237477 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
LJF87_RS05925 (LJF87_05890) 1237467..1238714 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
LJF87_RS05930 (LJF87_05895) 1238711..1239349 - 639 WP_011799158.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T220866 WP_003405820.1 NZ_CP085532:c1234225-1233914 [Mycobacterium tuberculosis variant bovis BCG]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T220866 NZ_CP117298:c2376250-2375933 [Mycobacterium tuberculosis variant bovis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT220866 WP_003405836.1 NZ_CP085532:c1234545-1234225 [Mycobacterium tuberculosis variant bovis BCG]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT220866 NZ_CP117298:c2376462-2376247 [Mycobacterium tuberculosis variant bovis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References