Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1026525..1027498 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name tacT Uniprot ID O05911
Locus tag LJF87_RS04880 Protein ID WP_003404756.1
Coordinates 1026998..1027498 (+) Length 167 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID G0TFR4
Locus tag LJF87_RS04875 Protein ID WP_003404754.1
Coordinates 1026525..1027001 (+) Length 159 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS04855 (LJF87_04830) 1022372..1023643 - 1272 WP_003404747.1 PPE family protein -
LJF87_RS04860 (LJF87_04835) 1023658..1023957 - 300 WP_003404748.1 PE family protein -
LJF87_RS04865 (LJF87_04840) 1024401..1026182 + 1782 WP_003404749.1 BCCT family transporter -
LJF87_RS04870 (LJF87_04845) 1026182..1026436 + 255 WP_003898646.1 hypothetical protein -
LJF87_RS04875 (LJF87_04850) 1026525..1027001 + 477 WP_003404754.1 DUF1778 domain-containing protein Antitoxin
LJF87_RS04880 (LJF87_04855) 1026998..1027498 + 501 WP_003404756.1 GNAT family N-acetyltransferase Toxin
LJF87_RS04890 (LJF87_04865) 1027811..1029130 - 1320 WP_010950468.1 IS256-like element IS1554 family transposase -
LJF87_RS04895 (LJF87_04870) 1029418..1029999 + 582 WP_003404760.1 IS607-like element IS1535 family transposase -
LJF87_RS04900 (LJF87_04875) 1029999..1031651 + 1653 WP_003404761.1 IS607 family element RNA-guided endonuclease TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1026179..1072092 45913


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(38-143)

Antitoxin

Vibrio phage ICP1, Orf50

(74-150)


Sequences

Toxin        


Download         Length: 167 a.a.        Molecular weight: 18048.55 Da        Isoelectric Point: 7.8659

>T220864 WP_003404756.1 NZ_CP085532:1026998-1027498 [Mycobacterium tuberculosis variant bovis BCG]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Download         Length: 501 bp

>T220864 NZ_CP117298:2298197-2298607 [Mycobacterium tuberculosis variant bovis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin


Download         Length: 159 a.a.        Molecular weight: 17357.95 Da        Isoelectric Point: 11.2467

>AT220864 WP_003404754.1 NZ_CP085532:1026525-1027001 [Mycobacterium tuberculosis variant bovis BCG]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Download         Length: 477 bp

>AT220864 NZ_CP117298:2297971-2298204 [Mycobacterium tuberculosis variant bovis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSZ2

Antitoxin

Source ID Structure
AlphaFold DB G0TFR4

References