Detailed information of TA system TA220863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1016995..1017614
• Replicon: chromosome
• Accession: NZ_CP085532
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LJF87_RS04825
• Protein ID: WP_003404726.1
• Coordinates: 1017180..1017614 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: LJF87_RS04820
• Protein ID: WP_003404724.1
• Coordinates: 1016995..1017174 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF87_RS04805 (LJF87_04780)	1012468..1014048	+	1581	WP_011799139.1	serine hydrolase	-
LJF87_RS04810 (LJF87_04785)	1014045..1016438	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
LJF87_RS04815 (LJF87_04790)	1016711..1016869	+	159	WP_003404720.1	hypothetical protein	-
LJF87_RS04820 (LJF87_04795)	1016995..1017174	+	180	WP_003404724.1	antitoxin	Antitoxin
LJF87_RS04825 (LJF87_04800)	1017180..1017614	+	435	WP_003404726.1	SRPBCC family protein	Toxin
LJF87_RS04830 (LJF87_04805)	1017712..1018485	+	774	WP_003404735.1	VOC family protein	-
LJF87_RS04835 (LJF87_04810)	1018550..1018999	+	450	WP_003404738.1	hypothetical protein	-
LJF87_RS04840 (LJF87_04815)	1019134..1019364	-	231	WP_003898642.1	hypothetical protein	-
LJF87_RS04845 (LJF87_04820)	1019531..1021039	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
LJF87_RS04850 (LJF87_04825)	1021041..1022279	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T220863 WP_003404726.1 NZ_CP085532:1017180-1017614 [Mycobacterium tuberculosis variant bovis BCG]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T220863 NZ_CP117298:2230413-2230811 [Mycobacterium tuberculosis variant bovis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT220863 WP_003404724.1 NZ_CP085532:1016995-1017174 [Mycobacterium tuberculosis variant bovis BCG]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT220863 NZ_CP117298:2230185-2230412 [Mycobacterium tuberculosis variant bovis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCGACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Antitoxin

• AlphaFold DB: G0TFQ5