Detailed information of TA system TA022086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 192085..192902
• Replicon: plasmid pACRY01
• Accession: NC_009467
• Organism: Acidiphilium cryptum JF-5

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: ACRY_RS18410
• Protein ID: WP_011930432.1
• Coordinates: 192085..192663 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: ACRY_RS19410
• Protein ID: WP_155856294.1
• Coordinates: 192660..192902 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACRY_RS00780	187721..188890	-	1170	WP_152982781.1	plasmid partitioning protein RepA	-
ACRY_RS00785	190843..191970	-	1128	WP_011930433.1	site-specific integrase	-
ACRY_RS18410	192085..192663	-	579	WP_011930432.1	PIN domain-containing protein	Toxin
ACRY_RS19410	192660..192902	-	243	WP_155856294.1	helix-turn-helix domain-containing protein	Antitoxin
ACRY_RS18050	193606..193869	-	264	WP_050751058.1	hypothetical protein	-
ACRY_RS00805	193868..194983	+	1116	WP_050751059.1	hypothetical protein	-
ACRY_RS00810	195252..195728	+	477	WP_011930428.1	serine hydrolase	-
ACRY_RS00815	195752..196462	+	711	WP_011930427.1	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..203589	203589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-115)

Antitoxin

• SinI-like, DNA-binding domain (11-56)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21984.28 Da
• Isoelectric Point: 6.6051

>T22086 WP_011930432.1 NC_009467:c192663-192085 [Acidiphilium cryptum JF-5]
MKHIADRFVVVLDANVLYPFRMRDILLCFYHAGLFRGRWSKSILEEWTSNLLAEKPGLHHSIRSQLQKMSFEFPEALVTG
YESLIGSLDLPDQNDRHVLAAAIRCGAQHIVTQNLNDFPPEALEPFSVEAIHPDEFLSRTFELYPTEATEILGKLRRSYQ
NPPYTPSEFVLDLTAKGLPKLASQIRPMRSFL

Nucleotide

• Length: 579 bp

>T22086 NC_009467:c192663-192085 [Acidiphilium cryptum JF-5]
ATGAAGCACATTGCGGACCGCTTTGTTGTCGTATTGGACGCAAATGTCCTGTATCCATTCAGGATGCGGGACATTCTTCT
GTGCTTCTATCATGCTGGCTTGTTTCGGGGCCGCTGGAGCAAATCAATATTGGAGGAATGGACTTCCAATCTCTTGGCTG
AAAAGCCAGGCTTGCACCACAGTATTCGTTCGCAGTTGCAAAAAATGAGTTTCGAGTTTCCAGAAGCGCTGGTTACCGGT
TACGAAAGCTTGATAGGCAGCCTCGACCTGCCTGATCAGAATGATCGGCACGTTCTCGCGGCTGCGATCCGCTGCGGAGC
ACAACACATCGTCACCCAAAATCTCAACGACTTCCCGCCAGAGGCTCTGGAGCCATTTTCTGTTGAAGCGATTCACCCCG
ATGAGTTTCTTTCCAGAACATTCGAGCTCTACCCCACAGAGGCAACCGAAATCCTCGGAAAGTTGAGACGCTCATATCAG
AATCCGCCGTACACTCCTTCAGAATTCGTGCTCGACCTCACAGCGAAAGGTCTCCCCAAACTTGCGTCGCAGATCCGTCC
GATGCGATCGTTTCTTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8938.19 Da
• Isoelectric Point: 7.0098

>AT22086 WP_155856294.1 NC_009467:c192902-192660 [Acidiphilium cryptum JF-5]
MVTFVPVGSMLTTQQAADLLNVSRPYLSGLLKNGEIPFVPIGSHRRVKFEDLIAYKNRRDSSRETALDELARLGQEFDAS

Nucleotide

• Length: 243 bp

>AT22086 NC_009467:c192902-192660 [Acidiphilium cryptum JF-5]
ATGGTAACCTTTGTTCCTGTCGGGTCGATGTTGACGACGCAACAGGCAGCCGATTTGCTCAACGTCTCGAGGCCATACCT
CAGCGGTCTGCTGAAGAACGGGGAGATCCCGTTTGTTCCAATCGGCTCACACAGGCGCGTTAAATTTGAAGATTTGATCG
CCTACAAAAATCGGCGTGATAGCAGTCGGGAAACTGCACTGGATGAATTGGCGCGCTTGGGCCAAGAATTCGATGCCTCA
TGA