Detailed information of TA system TA220847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0298-Rv0299/-
• Location: 364557..365083
• Replicon: chromosome
• Accession: NZ_CP085532
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: LJF87_RS01590
• Protein ID: WP_003401560.1
• Coordinates: 364781..365083 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: Rv0298
• Uniprot ID: P9WJ08
• Locus tag: LJF87_RS01585
• Protein ID: WP_003401555.1
• Coordinates: 364557..364784 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF87_RS01570 (LJF87_01570)	360205..361008	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
LJF87_RS01575 (LJF87_01575)	361018..362415	-	1398	WP_003401544.1	sulfatase	-
LJF87_RS01580 (LJF87_01580)	362594..364414	+	1821	WP_003401546.1	PE family protein	-
LJF87_RS01585 (LJF87_01585)	364557..364784	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	Antitoxin
LJF87_RS01590 (LJF87_01590)	364781..365083	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
LJF87_RS01595 (LJF87_01595)	365131..365352	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	-
LJF87_RS01600 (LJF87_01600)	365349..365774	+	426	WP_003401566.1	PIN domain nuclease	-
LJF87_RS01605 (LJF87_01605)	365911..366543	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
LJF87_RS01610 (LJF87_01610)	366540..367448	+	909	WP_003900117.1	protochlorophyllide reductase	-
LJF87_RS01615	367456..368046	-	591	WP_234712434.1	hypothetical protein	-
LJF87_RS01620	368039..368089	-	51	Protein_320	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T220847 WP_003401560.1 NZ_CP085532:364781-365083 [Mycobacterium tuberculosis variant bovis BCG]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T220847 NZ_CP117298:c1232623-1232312 [Mycobacterium tuberculosis variant bovis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8130.21 Da
• Isoelectric Point: 7.0084

>AT220847 WP_003401555.1 NZ_CP085532:364557-364784 [Mycobacterium tuberculosis variant bovis BCG]
MTKEKISVTVDAAVLAAIDADARAAGLNRSEMIEQALRNEHLRVALRDYTAKTVPALDIDAYAQRVYQANRAAGS

Nucleotide

• Length: 228 bp

>AT220847 NZ_CP117298:c1232943-1232623 [Mycobacterium tuberculosis variant bovis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Structures

Antitoxin

• AlphaFold DB: A0A7U4F8T1