220845

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 71618..72251 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name vapC Uniprot ID P9WFC0
Locus tag LJF87_RS00365 Protein ID WP_003400580.1
Coordinates 71850..72251 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7U4BR76
Locus tag LJF87_RS00360 Protein ID WP_011799070.1
Coordinates 71618..71857 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS00345 (LJF87_00345) 66915..68354 + 1440 WP_003901784.1 FAD-binding oxidoreductase -
LJF87_RS00350 (LJF87_00350) 68410..68571 + 162 WP_003899796.1 hypothetical protein -
LJF87_RS00355 (LJF87_00355) 68612..71551 + 2940 WP_003901785.1 UPF0182 family protein -
LJF87_RS00360 (LJF87_00360) 71618..71857 + 240 WP_011799070.1 hypothetical protein Antitoxin
LJF87_RS00365 (LJF87_00365) 71850..72251 + 402 WP_003400580.1 type II toxin-antitoxin system ribonuclease VapC1 Toxin
LJF87_RS00370 (LJF87_00370) 72303..74540 - 2238 WP_003400583.1 NADP-dependent isocitrate dehydrogenase -
LJF87_RS00375 (LJF87_00375) 74658..75227 - 570 WP_003400591.1 TetR/AcrR family transcriptional regulator -
LJF87_RS00380 (LJF87_00380) 75330..76241 + 912 WP_003899798.1 SDR family NAD(P)-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(36-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14339.44 Da        Isoelectric Point: 4.8887

>T220845 WP_003400580.1 NZ_CP085532:71850-72251 [Mycobacterium tuberculosis variant bovis BCG]
VDECVVDAAAVVDALAGKGASAIVLRGLLKESISNAPHLLDAEVGHALRRAVLSDEISEEQARAALDALPYLIDNRYPHS
PRLIEYTWQLRHNVTFYDALYVALATALDVPLLTGDSRLAAAPGLPCEIKLVR

Download         Length: 402 bp

>T220845 NZ_CP117298:1015395-1015829 [Mycobacterium tuberculosis variant bovis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8745.89 Da        Isoelectric Point: 8.5960

>AT220845 WP_011799070.1 NZ_CP085532:71618-71857 [Mycobacterium tuberculosis variant bovis BCG]
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEVIEILEQALASTASPGISRETIEASRRELRGG

Download         Length: 240 bp

>AT220845 NZ_CP117298:1015210-1015389 [Mycobacterium tuberculosis variant bovis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BR82

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BR76

References