Detailed information of TA system TA220843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 1390953..1391487
• Replicon: plasmid pSymB
• Accession: NZ_CP085528
• Organism: Sinorhizobium meliloti strain L6-AK89

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LJD24_RS31120
• Protein ID: WP_017270034.1
• Coordinates: 1390953..1391249 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: LJD24_RS31125
• Protein ID: WP_003527850.1
• Coordinates: 1391239..1391487 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJD24_RS31100 (LJD24_31100)	1387668..1388711	-	1044	WP_027988589.1	glycosyltransferase	-
LJD24_RS31105 (LJD24_31105)	1388742..1389131	-	390	WP_028006184.1	hypothetical protein	-
LJD24_RS31110 (LJD24_31110)	1389128..1390297	-	1170	WP_017269611.1	glycosyltransferase family 4 protein	-
LJD24_RS31115 (LJD24_31115)	1390619..1390879	+	261	Protein_1263	helix-turn-helix domain-containing protein	-
LJD24_RS31120 (LJD24_31120)	1390953..1391249	-	297	WP_017270034.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LJD24_RS31125 (LJD24_31125)	1391239..1391487	-	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
LJD24_RS31130 (LJD24_31130)	1391934..1393163	-	1230	WP_027988592.1	glycosyltransferase family 4 protein	-
LJD24_RS31135 (LJD24_31135)	1393174..1393929	-	756	WP_017270253.1	polysaccharide deacetylase family protein	-
LJD24_RS31140 (LJD24_31140)	1393922..1395253	-	1332	WP_028006217.1	O-antigen ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1645902	1645902

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-93)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11449.10 Da
• Isoelectric Point: 10.6593

>T220843 WP_017270034.1 NZ_CP085528:c1391249-1390953 [Sinorhizobium meliloti]
MATERPFRLAPAAKADLRKIWRYTARRWSLEQAETYQDQLYTAFEGLAGGTKKGRNVDVRPGYLKYPAGSHIVYFRDRGD
RIDIIRILHGRMDAQRHL

Nucleotide

• Length: 297 bp

>T220843 NZ_CP117298:c757219-756782 [Mycobacterium tuberculosis variant bovis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT220843 WP_003527850.1 NZ_CP085528:c1391487-1391239 [Sinorhizobium meliloti]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT220843 NZ_CP117298:c757470-757216 [Mycobacterium tuberculosis variant bovis]
ATGAGCATGCGTCTGGCGCATCGGCTCCAGATCCTGCTCGACGACGAGTGCCACCGCCGCATCACCGCGGTGGCGCGCGA
GCGAGGCGTCCCGGTCGCAACCGTCGTGCGTGAGGCCATTGATCGAGGCCTCGTCAGCCCCGCCGGTCGCCGCAAGTCCG
CGGGCAGGCGCCTGCTCGACGCCGCCGACATGTCCGTTCCCGAGCCGCGGGAGCTGAAGCAGGAACTCGAGGCCCTCCGG
GCGCGCCGCGGATGA

Structures

Antitoxin

• AlphaFold DB: H0FXK0