Detailed information of TA system TA220842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 737491..738423
• Replicon: plasmid pSymB
• Accession: NZ_CP085528
• Organism: Sinorhizobium meliloti strain L6-AK89

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q92VJ4
• Locus tag: LJD24_RS28230
• Protein ID: WP_010975576.1
• Coordinates: 737869..738423 (+)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: H0G7T7
• Locus tag: LJD24_RS28225
• Protein ID: WP_003534594.1
• Coordinates: 737491..737862 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJD24_RS28205 (LJD24_28205)	733378..733761	+	384	WP_010975573.1	RidA family protein	-
LJD24_RS28210 (LJD24_28210)	733778..735766	+	1989	WP_028006229.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-
LJD24_RS28215 (LJD24_28215)	735763..736632	+	870	WP_017269133.1	hydroxymethylglutaryl-CoA lyase	-
LJD24_RS28220 (LJD24_28220)	736629..737414	+	786	WP_017269132.1	crotonase/enoyl-CoA hydratase family protein	-
LJD24_RS28225 (LJD24_28225)	737491..737862	+	372	WP_003534594.1	DUF2384 domain-containing protein	Antitoxin
LJD24_RS28230 (LJD24_28230)	737869..738423	+	555	WP_010975576.1	RES family NAD+ phosphorylase	Toxin
LJD24_RS28235 (LJD24_28235)	738558..739928	-	1371	WP_017270898.1	FAD-binding oxidoreductase	-
LJD24_RS28240 (LJD24_28240)	740040..741143	-	1104	WP_028006228.1	sulfate/molybdate ABC transporter ATP-binding protein	-
LJD24_RS28245 (LJD24_28245)	741159..742034	-	876	WP_003537156.1	sulfate ABC transporter permease subunit CysW	-
LJD24_RS28250 (LJD24_28250)	742024..742890	-	867	WP_010975580.1	sulfate ABC transporter permease subunit CysT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1645902	1645902

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (23-177)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20459.35 Da
• Isoelectric Point: 6.4901

>T220842 WP_010975576.1 NZ_CP085528:737869-738423 [Sinorhizobium meliloti]
MRKHIPPASRPDPEPTSPVEPITLWRAFVPRWAHMPLSGAGAARFGGRWNPIGMPAIYAARELSTAWAEYNQGFVQHPAL
IVQLELRGARLADLTDASVLLELGVDEAIHRCEWRDALDKGAVPETHRLQSELLARDYHGVIYPSFMSPGGTCVALWRWN
GAEEPRLDVIDPDGRLPKSPASWV

Nucleotide

• Length: 555 bp

>T220842 NZ_CP117298:c757219-756782 [Mycobacterium tuberculosis variant bovis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13113.26 Da
• Isoelectric Point: 9.9996

>AT220842 WP_003534594.1 NZ_CP085528:737491-737862 [Sinorhizobium meliloti]
MMAVDFQIPAARFGDDHSPFLSARLVADRLGVTLAELAKLIGVARNTLTAKSGARKVDSALSKVVRILAMASEMAGDEAR
AVIWFKHQPIPGWAGKTAFDLVGEGKADKVLAYLESVRAGVYA

Nucleotide

• Length: 372 bp

>AT220842 NZ_CP117298:c756672-756427 [Mycobacterium tuberculosis variant bovis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: Q92VJ4

Antitoxin

• AlphaFold DB: H0G7T7