Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-Phd
Location 914062..914640 Replicon chromosome
Accession NC_009456
Organism Vibrio cholerae O395

Toxin (Protein)


Gene name parE Uniprot ID O68848
Locus tag VC0395_RS04445 Protein ID WP_001180243.1
Coordinates 914323..914640 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID Q7DCR7
Locus tag VC0395_RS04440 Protein ID WP_000557292.1
Coordinates 914062..914304 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VC0395_RS20805 909487..909525 - 39 WP_106019119.1 hypothetical protein -
VC0395_RS20065 909541..909666 - 126 WP_001944767.1 DUF645 family protein -
VC0395_RS20970 909843..909953 - 111 WP_134814058.1 DUF3265 domain-containing protein -
VC0395_RS04405 909950..910267 - 318 WP_001232701.1 HigA family addiction module antidote protein -
VC0395_RS04410 910278..910565 - 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin -
VC0395_RS04415 910832..911012 - 181 Protein_865 DUF645 family protein -
VC0395_RS04420 911437..911670 - 234 WP_032482776.1 DUF3709 domain-containing protein -
VC0395_RS04425 912073..912291 - 219 WP_001917086.1 DUF3709 domain-containing protein -
VC0395_RS04430 912601..913254 - 654 WP_000226874.1 hypothetical protein -
VC0395_RS04435 913311..913820 - 510 WP_000405986.1 GNAT family N-acetyltransferase -
VC0395_RS04440 914062..914304 + 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
VC0395_RS04445 914323..914640 + 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
VC0395_RS20975 914834..914929 - 96 WP_012706049.1 DUF645 family protein -
VC0395_RS04450 915388..916350 + 963 WP_000841995.1 integron integrase IntIA -
VC0395_RS04455 916432..916785 - 354 WP_001138366.1 50S ribosomal protein L20 -
VC0395_RS04460 916827..917021 - 195 WP_001885060.1 50S ribosomal protein L35 -
VC0395_RS04465 917125..917676 - 552 WP_001894072.1 translation initiation factor IF-3 -
VC0395_RS04470 917680..919608 - 1929 WP_001121783.1 threonine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integron - - 833458..916350 82892


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-93)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-71)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12158.97 Da        Isoelectric Point: 9.7495

>T22084 WP_001180243.1 NC_009456:914323-914640 [Vibrio cholerae O395]
MQNKQYKLSQLAQEHLLKIKHYTIENFAEAQWQKYKSTLLSGFQTLADNPGLGKSCEDIYQNGFYFPVGKHMAYYTKEAN
FILIVAVLGQSQLPQKHLKQSRFVS

Download         Length: 318 bp

>T22084 NC_009456:914323-914640 [Vibrio cholerae O395]
ATGCAAAATAAACAATATAAGCTCAGCCAATTAGCCCAAGAGCACTTACTCAAGATTAAACACTACACCATTGAAAATTT
CGCTGAAGCGCAGTGGCAAAAATATAAGTCGACCTTGCTTTCAGGTTTCCAAACTCTTGCGGATAACCCAGGACTAGGAA
AAAGCTGTGAAGATATTTACCAAAATGGTTTTTACTTTCCAGTGGGGAAACACATGGCTTATTACACCAAAGAAGCAAAC
TTCATTCTCATTGTTGCGGTATTAGGGCAATCACAACTGCCCCAAAAGCATCTCAAACAGTCACGCTTTGTTTCTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8961.25 Da        Isoelectric Point: 5.6630

>AT22084 WP_000557292.1 NC_009456:914062-914304 [Vibrio cholerae O395]
MHTLTANDAKRNFGELLLSAQREPVIISKNSKNTVVVMSIKDFEELEAMKLDYLKHCFESAQKDLDSGKTVDGATFLNTL

Download         Length: 243 bp

>AT22084 NC_009456:914062-914304 [Vibrio cholerae O395]
ATGCACACACTAACAGCAAATGATGCTAAACGAAATTTTGGTGAACTTCTTCTAAGCGCACAACGCGAACCTGTCATCAT
CAGCAAAAACAGTAAGAATACTGTCGTTGTCATGTCCATTAAGGACTTTGAAGAACTTGAAGCAATGAAACTCGATTATC
TCAAACACTGCTTTGAGTCTGCTCAGAAAGACTTAGATAGTGGCAAAACCGTTGATGGTGCCACTTTTCTAAACACCCTT
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K9UJR8

Antitoxin

Source ID Structure
AlphaFold DB Q7DCR7

References