Detailed information of TA system TA220838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 1114187..1114785
• Replicon: plasmid pSymA
• Accession: NZ_CP085527
• Organism: Sinorhizobium meliloti strain L6-AK89

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q930F0
• Locus tag: LJD24_RS24355
• Protein ID: WP_010967243.1
• Coordinates: 1114187..1114480 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LJD24_RS24360
• Protein ID: WP_014989717.1
• Coordinates: 1114477..1114785 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJD24_RS24340 (LJD24_24340)	1110406..1111182	+	777	WP_017270985.1	histidinol-phosphatase	-
LJD24_RS32365	1111560..1111817	-	258	WP_074853712.1	plasmid partitioning protein RepB C-terminal domain-containing protein	-
LJD24_RS24345 (LJD24_24345)	1111751..1112311	-	561	WP_234708620.1	plasmid partitioning protein RepB C-terminal domain-containing protein	-
LJD24_RS24350 (LJD24_24350)	1112726..1113925	+	1200	WP_227692170.1	NAD-dependent formate dehydrogenase	-
LJD24_RS24355 (LJD24_24355)	1114187..1114480	-	294	WP_010967243.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
LJD24_RS24360 (LJD24_24360)	1114477..1114785	-	309	WP_014989717.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LJD24_RS24365 (LJD24_24365)	1115428..1116912	-	1485	WP_017269058.1	hypothetical protein	-
LJD24_RS24370 (LJD24_24370)	1117182..1118084	-	903	WP_028006271.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1217792	1217792

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11278.93 Da
• Isoelectric Point: 6.4734

>T220838 WP_010967243.1 NZ_CP085527:c1114480-1114187 [Sinorhizobium meliloti]
MRLVWARYALDDRDTIFSYIERENPRAAVHVDEEIVSAVRRLLDFPESGRPGRIAGTRELVIPRTPYIAAYMVMEDRIRI
LRVLHGAQKWPSELDDG

Nucleotide

• Length: 294 bp

>T220838 NZ_CP117298:717588-717983 [Mycobacterium tuberculosis variant bovis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11228.61 Da
• Isoelectric Point: 5.6604

>AT220838 WP_014989717.1 NZ_CP085527:c1114785-1114477 [Sinorhizobium meliloti]
MASNALVQTRIDAEVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELFSHSEAHDAWFRAKVLRALEDTRPDVDDA
DADAHFRERRAAAMRKAAAGDR

Nucleotide

• Length: 309 bp

>AT220838 NZ_CP117298:717334-717588 [Mycobacterium tuberculosis variant bovis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Toxin

• AlphaFold DB: Q930F0