Detailed information of TA system TA220837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 847794..848293
• Replicon: plasmid pSymA
• Accession: NZ_CP085527
• Organism: Sinorhizobium meliloti strain L6-AK89

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q92YA6
• Locus tag: LJD24_RS23050
• Protein ID: WP_010967939.1
• Coordinates: 847794..847967 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A843ZXX9
• Locus tag: LJD24_RS23055
• Protein ID: WP_014990215.1
• Coordinates: 847964..848293 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJD24_RS23025 (LJD24_23025)	843659..844975	+	1317	WP_013845634.1	aspartate aminotransferase family protein	-
LJD24_RS23030 (LJD24_23030)	845354..845839	-	486	WP_010967936.1	YidB family protein	-
LJD24_RS23035 (LJD24_23035)	845867..846118	-	252	WP_003534243.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
LJD24_RS23040 (LJD24_23040)	846713..847072	+	360	WP_013845635.1	VOC family protein	-
LJD24_RS23045 (LJD24_23045)	847391..847696	+	306	WP_013845638.1	hypothetical protein	-
LJD24_RS23050 (LJD24_23050)	847794..847967	-	174	WP_010967939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LJD24_RS23055 (LJD24_23055)	847964..848293	-	330	WP_014990215.1	hypothetical protein	Antitoxin
LJD24_RS23060 (LJD24_23060)	848228..848413	+	186	WP_013845639.1	hypothetical protein	-
LJD24_RS23065 (LJD24_23065)	848654..850024	+	1371	WP_013845640.1	alpha/beta hydrolase	-
LJD24_RS23070 (LJD24_23070)	850408..850749	+	342	Protein_837	hypothetical protein	-
LJD24_RS23075 (LJD24_23075)	850733..851071	-	339	WP_013845641.1	SDR family oxidoreductase	-
LJD24_RS23080 (LJD24_23080)	851188..851631	-	444	WP_010967942.1	PACE efflux transporter	-
LJD24_RS23085 (LJD24_23085)	851723..852616	+	894	WP_010967943.1	LysR family transcriptional regulator	-
LJD24_RS23090 (LJD24_23090)	852742..852918	-	177	WP_013845643.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1217792	1217792

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6497.32 Da
• Isoelectric Point: 5.7733

>T220837 WP_010967939.1 NZ_CP085527:c847967-847794 [Sinorhizobium meliloti]
MKLVWARYALDDRDAIFSYIERENPRAAVHVDEEVVSAGRPLDFPESRRPGRIAGTP

Nucleotide

• Length: 174 bp

>T220837 NZ_CP117298:711929-712330 [Mycobacterium tuberculosis variant bovis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12067.72 Da
• Isoelectric Point: 11.1401

>AT220837 WP_014990215.1 NZ_CP085527:c848293-847964 [Sinorhizobium meliloti]
VSERLLLIALLALGPFRASQRHTGRPALLRRGPQTAAGAVEEHFIQLRAPLWLITLGGFSHREAHDAWFRAKVLRALEDT
RPDADDADADAHFQERRAAALRKAAAGDR

Nucleotide

• Length: 330 bp

>AT220837 NZ_CP117298:711705-711932 [Mycobacterium tuberculosis variant bovis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: Q92YA6

Antitoxin

• AlphaFold DB: A0A843ZXX9