Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/VapC-VagC |
| Location | 511543..512228 | Replicon | plasmid pSymA |
| Accession | NZ_CP085527 | ||
| Organism | Sinorhizobium meliloti strain L6-AK89 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | - |
| Locus tag | LJD24_RS21435 | Protein ID | WP_017263896.1 |
| Coordinates | 511543..511962 (-) | Length | 140 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | F7XET8 |
| Locus tag | LJD24_RS21440 | Protein ID | WP_013845417.1 |
| Coordinates | 511959..512228 (-) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LJD24_RS21405 (LJD24_21405) | 506822..507469 | + | 648 | WP_017270068.1 | autoinducer binding domain-containing protein | - |
| LJD24_RS21410 (LJD24_21410) | 507493..508178 | - | 686 | Protein_505 | ISNCY family transposase | - |
| LJD24_RS21415 (LJD24_21415) | 508538..509900 | - | 1363 | Protein_506 | ISNCY family transposase | - |
| LJD24_RS21420 (LJD24_21420) | 510170..510550 | - | 381 | WP_003526626.1 | transcriptional regulator | - |
| LJD24_RS21425 (LJD24_21425) | 510568..510879 | - | 312 | WP_026031097.1 | type II toxin-antitoxin system HigB family toxin | - |
| LJD24_RS21430 (LJD24_21430) | 511159..511389 | + | 231 | WP_017269009.1 | hypothetical protein | - |
| LJD24_RS21435 (LJD24_21435) | 511543..511962 | - | 420 | WP_017263896.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| LJD24_RS21440 (LJD24_21440) | 511959..512228 | - | 270 | WP_013845417.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| LJD24_RS21445 (LJD24_21445) | 512366..512859 | + | 494 | Protein_512 | LysR substrate-binding domain-containing protein | - |
| LJD24_RS21450 (LJD24_21450) | 512947..513822 | - | 876 | WP_013845418.1 | LysR family transcriptional regulator | - |
| LJD24_RS21455 (LJD24_21455) | 514572..515042 | + | 471 | WP_013845338.1 | transposase | - |
| LJD24_RS21460 (LJD24_21460) | 515039..515395 | + | 357 | WP_013845337.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| LJD24_RS21465 (LJD24_21465) | 515444..517018 | + | 1575 | WP_028006275.1 | IS66 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | IScluster/Tn | - | - | 507495..517578 | 10083 | |
| - | inside | Conjugative plasmid | - | htpB | 1..1217792 | 1217792 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 140 a.a. Molecular weight: 14732.00 Da Isoelectric Point: 4.8722
>T220836 WP_017263896.1 NZ_CP085527:c511962-511543 [Sinorhizobium meliloti]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSG
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSG
Download Length: 420 bp
>T220836 NZ_CP117298:704409-704810 [Mycobacterium tuberculosis variant bovis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA
Antitoxin
Download Length: 90 a.a. Molecular weight: 10203.67 Da Isoelectric Point: 4.4798
>AT220836 WP_013845417.1 NZ_CP085527:c512228-511959 [Sinorhizobium meliloti]
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL
Download Length: 270 bp
>AT220836 NZ_CP117298:704167-704412 [Mycobacterium tuberculosis variant bovis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|