Detailed information of TA system TA220835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 165859..166439
• Replicon: plasmid pSymA
• Accession: NZ_CP085527
• Organism: Sinorhizobium meliloti strain L6-AK89

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LJD24_RS19735
• Protein ID: WP_017270978.1
• Coordinates: 165859..166242 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XCL0
• Locus tag: LJD24_RS19740
• Protein ID: WP_013851145.1
• Coordinates: 166239..166439 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJD24_RS19710 (LJD24_19710)	161191..161685	-	495	WP_017269444.1	DUF4411 family protein	-
LJD24_RS19715 (LJD24_19715)	161682..162920	-	1239	WP_227692172.1	XRE family transcriptional regulator	-
LJD24_RS19720 (LJD24_19720)	163338..164537	-	1200	WP_014989784.1	NAD-dependent formate dehydrogenase	-
LJD24_RS19725 (LJD24_19725)	164880..165704	+	825	Protein_167	ParB N-terminal domain-containing protein	-
LJD24_RS19735 (LJD24_19735)	165859..166242	-	384	WP_017270978.1	type II toxin-antitoxin system VapC family toxin	Toxin
LJD24_RS19740 (LJD24_19740)	166239..166439	-	201	WP_013851145.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LJD24_RS19745 (LJD24_19745)	166955..167482	+	528	WP_080590796.1	TIGR02444 family protein	-
LJD24_RS19750 (LJD24_19750)	167673..168446	+	774	WP_017268955.1	UTRA domain-containing protein	-
LJD24_RS19755 (LJD24_19755)	168535..169911	-	1377	WP_014989788.1	FAD/NAD(P)-binding oxidoreductase	-
LJD24_RS19760 (LJD24_19760)	169908..170222	-	315	WP_014528248.1	(2Fe-2S)-binding protein	-
LJD24_RS19765 (LJD24_19765)	170212..171318	-	1107	WP_014989789.1	FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1217792	1217792

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14274.50 Da
• Isoelectric Point: 6.9172

>T220835 WP_017270978.1 NZ_CP085527:c166242-165859 [Sinorhizobium meliloti]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSI
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Nucleotide

• Length: 384 bp

>T220835 NZ_CP117298:c698490-698077 [Mycobacterium tuberculosis variant bovis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7096.22 Da
• Isoelectric Point: 11.1107

>AT220835 WP_013851145.1 NZ_CP085527:c166439-166239 [Sinorhizobium meliloti]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT220835 NZ_CP117298:c698723-698487 [Mycobacterium tuberculosis variant bovis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7W6MID2