Detailed information of TA system TA220832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2920100..2920668
• Replicon: chromosome
• Accession: NZ_CP085525
• Organism: Sinorhizobium meliloti strain L6-AK89

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: LJD24_RS14090
• Protein ID: WP_028006149.1
• Coordinates: 2920100..2920345 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: LJD24_RS14095
• Protein ID: WP_028006150.1
• Coordinates: 2920345..2920668 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJD24_RS14065 (LJD24_14065)	2915544..2915783	-	240	WP_003533366.1	hypothetical protein	-
LJD24_RS14070 (LJD24_14070)	2915780..2916001	-	222	WP_028006146.1	hypothetical protein	-
LJD24_RS14075 (LJD24_14075)	2916001..2918058	-	2058	WP_028006147.1	phage portal protein	-
LJD24_RS14080 (LJD24_14080)	2918058..2919563	-	1506	WP_050577110.1	hypothetical protein	-
LJD24_RS14085 (LJD24_14085)	2919541..2919999	-	459	WP_028006148.1	hypothetical protein	-
LJD24_RS14090 (LJD24_14090)	2920100..2920345	+	246	WP_028006149.1	BrnT family toxin	Toxin
LJD24_RS14095 (LJD24_14095)	2920345..2920668	+	324	WP_028006150.1	BrnA antitoxin family protein	Antitoxin
LJD24_RS14100 (LJD24_14100)	2920805..2921005	-	201	WP_028006151.1	hypothetical protein	-
LJD24_RS14105 (LJD24_14105)	2921005..2921163	-	159	WP_202902543.1	hypothetical protein	-
LJD24_RS14110 (LJD24_14110)	2921478..2922017	+	540	WP_155247946.1	hypothetical protein	-
LJD24_RS14115 (LJD24_14115)	2922052..2922729	-	678	WP_033043874.1	DUF6362 family protein	-
LJD24_RS14120 (LJD24_14120)	2922701..2923120	-	420	WP_028006154.1	hypothetical protein	-
LJD24_RS14125 (LJD24_14125)	2923240..2923932	+	693	WP_155247947.1	hypothetical protein	-
LJD24_RS14130 (LJD24_14130)	2924044..2924652	-	609	WP_080661613.1	ribonuclease H	-
LJD24_RS14135 (LJD24_14135)	2924717..2925100	-	384	WP_028006156.1	hypothetical protein	-
LJD24_RS14140 (LJD24_14140)	2925097..2925378	-	282	WP_028006157.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2870624..2927853	57229

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-101)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 8995.50 Da
• Isoelectric Point: 5.8235

>T220832 WP_028006149.1 NZ_CP085525:2920100-2920345 [Sinorhizobium meliloti]
MEIIWDEPKRIANIEKHGLDFADLSLEFFLASLVVPAKNGRSKAIGRLEDGTIVVIFVTLGSEALSVISMRPARKDERSL
L

Nucleotide

• Length: 246 bp

>T220832 NZ_CP117298:c445675-445082 [Mycobacterium tuberculosis variant bovis]
GTGAAGCGGCTCGATCTGGTCGCCGGGCCCAACGGCGCCGGCAAGTCGACGTTCGTCGCCCTCACGCTGGCGCCCTTGCT
GCCCGGCATCGTCTTCGTCAACGCCGACGAAATCGCCAAACAACGCTGGCCCGACGACCCAACATCGCACGCCTACCAGG
CGGCGCAGGTCGCCGCCGACACCCGCGCGAGGCTCATCGACTTGGGCCGGCCGTTCATTGCCGAGACGGTGTTCTCGCAC
CCATCGAAGCTCGAGCTCATCCGCACCGCGCGCACGGCCGGCTACACCGTCGTACTGCACGTGTTGGTTATCCCCGAAGG
CCTGGCGGTCGAGCGCGTCAGGCATCGCGTCGCCGCGGGCGGCCACGATGTGCCGGAGACCAAGATCCGCGAGCGTCACC
GCCGGCTGGCCGAGCTTGTCGCCCAGGCCATCACGCTGGCCGACGGCGCCACCGTCTATGACAACAGCCGGCTCGCGGGC
CCGCGGATCGTCGCTCAGTTCAGCGGGGGTGGCATCATCGGGCGCGCATGCTGGCCTTCGTGGACGCCGCCACCCTTGAT
GTCACGCTGGAGTAACAGGCCTGAGACGGCGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12173.75 Da
• Isoelectric Point: 7.0185

>AT220832 WP_028006150.1 NZ_CP085525:2920345-2920668 [Sinorhizobium meliloti]
MAITYKRLEEFQPGRGYSKEDWDAVSDNPPLTEEELKQARPFKEAFPDIAEEIEKAVRGRPKADLTKTPVTIRLDPDIVD
HYKATGKGWQSRMNDDLRKAAGLKKAS

Nucleotide

• Length: 324 bp

>AT220832 NZ_CP117298:c446093-445704 [Mycobacterium tuberculosis variant bovis]
GTGCCCAAAGCCGTCGACCGCGTCACGCGCGTCGCCGCGGATCTGGTGGACAGCGCGGCGGCCGAGGGTGCTCGTCAGAG
CCGTTCGGCCAAGCAGCAGCTCGACCACTGGGCCCGCGTCGGTCGGGCGGTGTCGAACCAGCACACGGCGTCGCGGCGCC
GCGTGGAAGCTGCGCTCGCGGGGCATCTGCCGATGACGGACCTGACGCTGGAGGAGGGCGTGGTGTTCAACGCCGAGATC
TCCGCAGCTATCGAGGAGCGCCTGTCGCGAACCAACTACGGCGACGTGCTCGCCGCGCAGGGCATCACCACGGTCGCCCT
GAACGACGCGGGCGACATCGTCGAGCACCGGCCAGACGGCACCTCGGTCGTGCTGGCGGCCACGCCGTGA