Detailed information of TA system TA220831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2862551..2863182
• Replicon: chromosome
• Accession: NZ_CP085525
• Organism: Sinorhizobium meliloti strain L6-AK89

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LJD24_RS13770
• Protein ID: WP_013844828.1
• Coordinates: 2862551..2862949 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92MD9
• Locus tag: LJD24_RS13775
• Protein ID: WP_010970129.1
• Coordinates: 2862949..2863182 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJD24_RS13735 (LJD24_13735)	2857630..2857932	-	303	WP_010970122.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LJD24_RS13740 (LJD24_13740)	2857929..2858204	-	276	WP_010970123.1	type II toxin-antitoxin system ParD family antitoxin	-
LJD24_RS13745 (LJD24_13745)	2858493..2858642	+	150	WP_010970124.1	hypothetical protein	-
LJD24_RS13750 (LJD24_13750)	2858639..2859574	+	936	WP_010970125.1	site-specific tyrosine recombinase XerD	-
LJD24_RS13755 (LJD24_13755)	2859638..2860591	+	954	WP_003527479.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
LJD24_RS13760 (LJD24_13760)	2860836..2862278	+	1443	WP_028006118.1	murein L,D-transpeptidase family protein	-
LJD24_RS13765 (LJD24_13765)	2862278..2862517	+	240	WP_010970127.1	sulfurtransferase TusA family protein	-
LJD24_RS13770 (LJD24_13770)	2862551..2862949	-	399	WP_013844828.1	type II toxin-antitoxin system toxin VapC	Toxin
LJD24_RS13775 (LJD24_13775)	2862949..2863182	-	234	WP_010970129.1	type II toxin-antitoxin system antitoxin VapB	Antitoxin
LJD24_RS13780 (LJD24_13780)	2863291..2864436	-	1146	WP_010970130.1	GTP-binding protein	-
LJD24_RS13785 (LJD24_13785)	2864450..2865532	-	1083	WP_017268305.1	D-alanyl-D-alanine carboxypeptidase family protein	-
LJD24_RS13790 (LJD24_13790)	2865784..2866947	+	1164	WP_010970132.1	M20 aminoacylase family protein	-
LJD24_RS13795 (LJD24_13795)	2866970..2867434	+	465	WP_010970133.1	Lrp/AsnC ligand binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14829.13 Da
• Isoelectric Point: 6.9581

>T220831 WP_013844828.1 NZ_CP085525:c2862949-2862551 [Sinorhizobium meliloti]
MLTYMLDTNICIYVMKTYPPAVREKFNGLAEQLCISSITLGELHYGAEKSARRVENLTAIEHFVARLEVLPFADKAAAHY
GQVRAELERTGTPCGPHDMQIGAHARSEGLIVVTNNIREFVRMPGVRVENWL

Nucleotide

• Length: 399 bp

>T220831 NZ_CP117298:364933-365358 [Mycobacterium tuberculosis variant bovis]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8673.87 Da
• Isoelectric Point: 8.9167

>AT220831 WP_010970129.1 NZ_CP085525:c2863182-2862949 [Sinorhizobium meliloti]
MASSTVFISNRSQAVRLPKAVAFPEGVHQVDILKIGRSRVIVPQGKRWDDLFLSGPRVSEDFMSERDQPVAETRESF

Nucleotide

• Length: 234 bp

>AT220831 NZ_CP117298:364715-364936 [Mycobacterium tuberculosis variant bovis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• AlphaFold DB: Q92MD9