Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacT-ataR/DUF1778(antitoxin)
Location 2815001..2815767 Replicon chromosome
Accession NZ_CP085525
Organism Sinorhizobium meliloti strain L6-AK89

Toxin (Protein)


Gene name tacT Uniprot ID H0G6D3
Locus tag LJD24_RS13500 Protein ID WP_003533488.1
Coordinates 2815285..2815767 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID H0G6D4
Locus tag LJD24_RS13495 Protein ID WP_003533490.1
Coordinates 2815001..2815285 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJD24_RS13480 (LJD24_13480) 2810444..2811859 + 1416 WP_014529872.1 lipopolysaccharide biosynthesis protein -
LJD24_RS13485 (LJD24_13485) 2811863..2813134 + 1272 WP_010970087.1 GNAT family N-acetyltransferase -
LJD24_RS13490 (LJD24_13490) 2813150..2814796 - 1647 WP_010970088.1 AMP-binding protein -
LJD24_RS13495 (LJD24_13495) 2815001..2815285 + 285 WP_003533490.1 DUF1778 domain-containing protein Antitoxin
LJD24_RS13500 (LJD24_13500) 2815285..2815767 + 483 WP_003533488.1 GNAT family N-acetyltransferase Toxin
LJD24_RS13510 (LJD24_13510) 2816232..2816741 + 510 WP_013844819.1 disulfide bond formation protein B -
LJD24_RS13515 (LJD24_13515) 2816773..2817330 - 558 WP_003533486.1 HNH endonuclease -
LJD24_RS13520 (LJD24_13520) 2817436..2818083 - 648 WP_010970091.1 DNA-3-methyladenine glycosylase -
LJD24_RS13525 (LJD24_13525) 2818236..2819132 + 897 WP_010970092.1 tRNA glutamyl-Q(34) synthetase GluQRS -
LJD24_RS13530 (LJD24_13530) 2819151..2820026 - 876 WP_010970093.1 YihY/virulence factor BrkB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-142)

Antitoxin

Vibrio phage ICP1, Orf50

(1-92)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17463.88 Da        Isoelectric Point: 7.3827

>T220830 WP_003533488.1 NZ_CP085525:2815285-2815767 [Sinorhizobium meliloti]
MLSAPTPLGEAHDFDLFQSGNDTLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Download         Length: 483 bp

>T220830 NZ_CP117298:364365-364667 [Mycobacterium tuberculosis variant bovis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10771.12 Da        Isoelectric Point: 7.3175

>AT220830 WP_003533490.1 NZ_CP085525:2815001-2815285 [Sinorhizobium meliloti]
MATKSSTDTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Download         Length: 285 bp

>AT220830 NZ_CP117298:364715-364936 [Mycobacterium tuberculosis variant bovis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H0G6D3

Antitoxin

Source ID Structure
AlphaFold DB H0G6D4

References