Detailed information of TA system TA220823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-ParD
• Location: 3281484..3282112
• Replicon: chromosome
• Accession: NZ_CP085524
• Organism: Zhaonella formicivorans strain K32

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EYS13_RS15985
• Protein ID: WP_227764682.1
• Coordinates: 3281762..3282112 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: EYS13_RS15980
• Protein ID: WP_227764678.1
• Coordinates: 3281484..3281759 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYS13_RS15960	3276621..3277499	+	879	WP_227764671.1	phosphate ABC transporter substrate-binding protein	-
EYS13_RS15965	3277671..3278039	+	369	WP_227764673.1	holo-ACP synthase	-
EYS13_RS15970	3278052..3279656	+	1605	WP_227764674.1	NAD(P)H-hydrate dehydratase	-
EYS13_RS15975	3279977..3281092	+	1116	WP_227764676.1	alanine racemase	-
EYS13_RS15980	3281484..3281759	+	276	WP_227764678.1	ribbon-helix-helix protein, CopG family	Antitoxin
EYS13_RS15985	3281762..3282112	+	351	WP_227764682.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EYS13_RS15990	3282304..3283014	+	711	WP_227764684.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
EYS13_RS15995	3283094..3283939	+	846	WP_227764686.1	NGG1p interacting factor NIF3	-
EYS13_RS16000	3283939..3285096	+	1158	WP_227764688.1	amidohydrolase	-
EYS13_RS16005	3285175..3285540	+	366	WP_227764689.1	response regulator	-
EYS13_RS16010	3285794..3286273	+	480	WP_227764692.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
EYS13_RS16015	3286254..3286964	+	711	WP_227764694.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-42)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12903.93 Da
• Isoelectric Point: 5.8834

>T220823 WP_227764682.1 NZ_CP085524:3281762-3282112 [Zhaonella formicivorans]
MYIRRGDIFFAQLNPVVGSEQGGMRPVLIVQNDIGNQYSPTTIVAAITSQIFKAKLPTHVEISAEKSGLEKDSVILTEQI
RTIDKSRLKQKVAFLDEETMEKVNKAIEISLGLTEI

Nucleotide

• Length: 351 bp

>T220823 NZ_CP117294:c4126911-4126423 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATCTCCGCCCCTGAACCGCTTCATGCCGGGCATATTCTTACCCCCTTCTGCTGCGGTGTGGATTCAATGGATAACTG
GCTAAAACAGCGGGCGATGAAAAATCAGGTAACTGGCGCATCCCGTACCTTTGTGTGTTGCGGCAGCGATTCTGACGTAC
TGGCGTATTATTCGCTGGCGTCCAGCGCGGTAACGACAAACACCGCCCCCGGTCGCTTTCGTCGCAATATGCCCGATCCG
ATCCCGGTTGTGGTGCTGGGCCGTCTGGCGGTGGATCGGTCGCTACATGGGCAGGGCGTAGGTCGGGCGCTGGTGAGGGA
TGCCGGGCTGCGGGTGATTCAGGTGGCGGAAACTATCGGTATTCGCGGGATGCTGGTCCATGCCCTGTCGGATGAAGCGC
GGGAGTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTT
CATGCCTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10852.59 Da
• Isoelectric Point: 6.3946

>AT220823 WP_227764678.1 NZ_CP085524:3281484-3281759 [Zhaonella formicivorans]
MSGVKRIMISIPESLLKEVDGLVSLEKRNRSEFIREAMRLYISERKRRTVREQMKKGYQEMAQINLALAMESYELEDEVQ
TYYDEKLVECK

Nucleotide

• Length: 276 bp

>AT220823 NZ_CP117294:c4127201-4126908 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCAGTCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGCATCAAGCCCGCCGAGCGCGATCTTATCGA
TCGGGCTGCAAAAGCCAGAGGGAAAAACTGCACAGACTTTGTGCTAGAAGCTGCCCGCGCCGCCGCCGAGGAAGCGCTGA
TCGAGCAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGCATTTCTGATCCGTCTGGATCAGGCTCCTGCGCCAAAC
GATGCGTTGCGTAAAACCATGCAGACTCCTGCACCGTGGGAGCAGGAAAAATGA