Detailed information of TA system TA220820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 272854..273493
• Replicon: chromosome
• Accession: NZ_CP085514
• Organism: Peribacillus frigoritolerans strain KF19

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LIT31_RS01410
• Protein ID: WP_034306610.1
• Coordinates: 273143..273493 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: LIT31_RS01405
• Protein ID: WP_034306380.1
• Coordinates: 272854..273135 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT31_RS01385 (LIT31_01385)	268993..269583	-	591	WP_048682037.1	rhomboid family intramembrane serine protease	-
LIT31_RS01390 (LIT31_01390)	269691..270041	+	351	WP_252279509.1	holo-ACP synthase	-
LIT31_RS01395 (LIT31_01395)	270284..271288	+	1005	WP_252279510.1	outer membrane lipoprotein carrier protein LolA	-
LIT31_RS01400 (LIT31_01400)	271506..272693	+	1188	WP_252279511.1	alanine racemase	-
LIT31_RS01405 (LIT31_01405)	272854..273135	+	282	WP_034306380.1	antitoxin EndoAI	Antitoxin
LIT31_RS01410 (LIT31_01410)	273143..273493	+	351	WP_034306610.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LIT31_RS01415 (LIT31_01415)	273907..274734	+	828	WP_048682050.1	RsbT co-antagonist protein RsbRA	-
LIT31_RS01420 (LIT31_01420)	274737..275093	+	357	WP_061143976.1	STAS domain-containing protein	-
LIT31_RS01425 (LIT31_01425)	275097..275498	+	402	WP_252279512.1	anti-sigma regulatory factor	-
LIT31_RS01430 (LIT31_01430)	275508..276518	+	1011	WP_252279513.1	PP2C family protein-serine/threonine phosphatase	-
LIT31_RS01435 (LIT31_01435)	276578..276910	+	333	WP_034306369.1	anti-sigma factor antagonist	-
LIT31_RS01440 (LIT31_01440)	276907..277380	+	474	WP_034306366.1	anti-sigma B factor RsbW	-
LIT31_RS01445 (LIT31_01445)	277358..278146	+	789	WP_034306363.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12955.06 Da
• Isoelectric Point: 7.2029

>T220820 WP_034306610.1 NZ_CP085514:273143-273493 [Peribacillus frigoritolerans]
IVVKRGDVYFADLSPVVGSEQGGTRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEPMMQKVNEALQISLGLIEF

Nucleotide

• Length: 351 bp

>T220820 NZ_CP117294:c2778324-2778222 [Salmonella enterica subsp. enterica serovar Typhi]
GCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10713.13 Da
• Isoelectric Point: 5.9054

>AT220820 WP_034306380.1 NZ_CP085514:272854-273135 [Peribacillus frigoritolerans]
VSESSATREILIRLPQNFLTELDGYASEENVNRSEFIYRATKMYLRERKKKEFRESMKRGYIEMAAINLTIASEAFQAEF
EAGHCVERLVSGG

Nucleotide

• Length: 282 bp

>AT220820 NZ_CP117294:2778220-2778363 [Salmonella enterica subsp. enterica serovar Typhi]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCTCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG