Detailed information of TA system TA220819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2307142..2308120
• Replicon: chromosome
• Accession: NZ_CP085510
• Organism: Bacillus cereus strain HD1.4B

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: HQJ03_RS11935
• Protein ID: WP_000624977.1
• Coordinates: 2307142..2307879 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: HQJ03_RS11940
• Protein ID: WP_000237819.1
• Coordinates: 2307992..2308120 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQJ03_RS11915 (HQJ03_011915)	2302784..2303566	+	783	WP_000355673.1	class I SAM-dependent methyltransferase	-
HQJ03_RS11920 (HQJ03_011920)	2303724..2305400	-	1677	WP_173602634.1	alpha-keto acid decarboxylase family protein	-
HQJ03_RS11925 (HQJ03_011925)	2305517..2305999	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
HQJ03_RS11930 (HQJ03_011930)	2306166..2306903	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
HQJ03_RS11935 (HQJ03_011935)	2307142..2307879	+	738	WP_000624977.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HQJ03_RS11940 (HQJ03_011940)	2307992..2308120	+	129	WP_000237819.1	hypothetical protein	Antitoxin
HQJ03_RS11945 (HQJ03_011945)	2308193..2308369	+	177	WP_000852629.1	stage II sporulation protein SB	-
HQJ03_RS11950 (HQJ03_011950)	2308387..2308776	-	390	WP_000713869.1	YxeA family protein	-
HQJ03_RS11955 (HQJ03_011955)	2308988..2310457	+	1470	WP_000287546.1	beta-Ala-His dipeptidase	-
HQJ03_RS11960 (HQJ03_011960)	2310703..2311512	+	810	WP_000664544.1	papain-like cysteine protease family protein	-
HQJ03_RS11965 (HQJ03_011965)	2311538..2312140	+	603	WP_000517259.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T220819 WP_000624977.1 NZ_CP085510:2307142-2307879 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T220819 NZ_CP117294:c1390751-1390533 [Salmonella enterica subsp. enterica serovar Typhi]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT220819 WP_000237819.1 NZ_CP085510:2307992-2308120 [Bacillus cereus]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT220819 NZ_CP117294:c1391153-1390779 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2