Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 236475..237117 Replicon chromosome
Accession NZ_CP085510
Organism Bacillus cereus strain HD1.4B

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag HQJ03_RS01360 Protein ID WP_000635963.1
Coordinates 236767..237117 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag HQJ03_RS01355 Protein ID WP_000004570.1
Coordinates 236475..236762 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HQJ03_RS01330 (HQJ03_001330) 231792..232754 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
HQJ03_RS01335 (HQJ03_001335) 232747..233319 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
HQJ03_RS01340 (HQJ03_001340) 233413..233772 + 360 WP_000583416.1 holo-ACP synthase -
HQJ03_RS01345 (HQJ03_001345) 233929..234879 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
HQJ03_RS01350 (HQJ03_001350) 234997..236166 + 1170 WP_000390615.1 alanine racemase -
HQJ03_RS01355 (HQJ03_001355) 236475..236762 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
HQJ03_RS01360 (HQJ03_001360) 236767..237117 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HQJ03_RS01365 (HQJ03_001365) 237185..239353 + 2169 WP_000426242.1 Tex family protein -
HQJ03_RS01370 (HQJ03_001370) 239411..239527 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
HQJ03_RS01375 (HQJ03_001375) 239723..240181 + 459 WP_000344231.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T220818 WP_000635963.1 NZ_CP085510:236767-237117 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T220818 NZ_CP117294:c976423-975896 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACAAAAACCTACGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT220818 WP_000004570.1 NZ_CP085510:236475-236762 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT220818 NZ_CP117294:c976710-976420 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGATTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References