Detailed information of TA system TA220817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2306609..2307587
• Replicon: chromosome
• Accession: NZ_CP085506
• Organism: Bacillus cereus strain HD2.4

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: HQG80_RS11920
• Protein ID: WP_000624977.1
• Coordinates: 2306609..2307346 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: HQG80_RS11925
• Protein ID: WP_000237819.1
• Coordinates: 2307459..2307587 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQG80_RS11900 (HQG80_011900)	2302251..2303033	+	783	WP_000355673.1	class I SAM-dependent methyltransferase	-
HQG80_RS11905 (HQG80_011905)	2303191..2304867	-	1677	WP_173602634.1	alpha-keto acid decarboxylase family protein	-
HQG80_RS11910 (HQG80_011910)	2304984..2305466	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
HQG80_RS11915 (HQG80_011915)	2305633..2306370	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
HQG80_RS11920 (HQG80_011920)	2306609..2307346	+	738	WP_000624977.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HQG80_RS11925 (HQG80_011925)	2307459..2307587	+	129	WP_000237819.1	hypothetical protein	Antitoxin
HQG80_RS11930 (HQG80_011930)	2307660..2307836	+	177	WP_000852629.1	stage II sporulation protein SB	-
HQG80_RS11935 (HQG80_011935)	2307854..2308243	-	390	WP_000713869.1	YxeA family protein	-
HQG80_RS11940 (HQG80_011940)	2308455..2309924	+	1470	WP_000287546.1	beta-Ala-His dipeptidase	-
HQG80_RS11945 (HQG80_011945)	2310170..2310979	+	810	WP_000664544.1	papain-like cysteine protease family protein	-
HQG80_RS11950 (HQG80_011950)	2311005..2311607	+	603	WP_000517259.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T220817 WP_000624977.1 NZ_CP085506:2306609-2307346 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T220817 NZ_CP117294:822597-822995 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCTGAAATTCATGCTTGATACCAATACTTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCACCGG
AGCGTAATTTTGCAGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTACCGAACTGGCCCGCAAGGGAACACCTGTCGGGTCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT220817 WP_000237819.1 NZ_CP085506:2307459-2307587 [Bacillus cereus]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT220817 NZ_CP117294:822370-822597 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGCACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2