Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 3014713..3015355 Replicon chromosome
Accession NZ_CP085501
Organism Bacillus cereus strain A24

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag FHQ13_RS15560 Protein ID WP_000635965.1
Coordinates 3015005..3015355 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FHQ13_RS15555 Protein ID WP_000004570.1
Coordinates 3014713..3015000 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FHQ13_RS15530 (FHQ13_015530) 3010030..3010992 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
FHQ13_RS15535 (FHQ13_015535) 3010985..3011557 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
FHQ13_RS15540 (FHQ13_015540) 3011651..3012010 + 360 WP_000583415.1 holo-ACP synthase -
FHQ13_RS15545 (FHQ13_015545) 3012167..3013117 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
FHQ13_RS15550 (FHQ13_015550) 3013235..3014404 + 1170 WP_000390615.1 alanine racemase -
FHQ13_RS15555 (FHQ13_015555) 3014713..3015000 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FHQ13_RS15560 (FHQ13_015560) 3015005..3015355 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FHQ13_RS15565 (FHQ13_015565) 3015423..3017591 + 2169 WP_000426236.1 Tex family protein -
FHQ13_RS15570 (FHQ13_015570) 3017649..3017765 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FHQ13_RS15575 (FHQ13_015575) 3017961..3018419 + 459 WP_000344237.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T220810 WP_000635965.1 NZ_CP085501:3015005-3015355 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T220810 NZ_CP117293:c4008048-4007764 [Salmonella enterica subsp. enterica serovar Typhi]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT220810 WP_000004570.1 NZ_CP085501:3014713-3015000 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT220810 NZ_CP117293:c4008280-4008038 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References