Detailed information of TA system TA220804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3429225..3430062
• Replicon: chromosome
• Accession: NZ_CP085488
• Organism: Escherichia coli strain VCSW8

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: LJY68_RS16520
• Protein ID: WP_000227784.1
• Coordinates: 3429520..3430062 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: LJY68_RS16515
• Protein ID: WP_001297137.1
• Coordinates: 3429225..3429536 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJY68_RS16490 (3424245)	3424245..3425192	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
LJY68_RS16495 (3425214)	3425214..3427205	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
LJY68_RS16500 (3427195)	3427195..3427809	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
LJY68_RS16505 (3427809)	3427809..3428138	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
LJY68_RS16510 (3428150)	3428150..3429040	+	891	WP_000971336.1	heme o synthase	-
LJY68_RS16515 (3429225)	3429225..3429536	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
LJY68_RS16520 (3429520)	3429520..3430062	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
LJY68_RS16525 (3430118)	3430118..3431053	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
LJY68_RS16530 (3431461)	3431461..3432825	+	1365	WP_001000978.1	MFS transporter	-
LJY68_RS16535 (3432953)	3432953..3433444	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
LJY68_RS16540 (3433612)	3433612..3434523	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T220804 WP_000227784.1 NZ_CP085488:3429520-3430062 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T220804 NZ_CP117293:805862-806263 [Salmonella enterica subsp. enterica serovar Typhi]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCCTTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGACAG
GACTGGACGCTCTTGCGTCCTCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
AG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT220804 WP_001297137.1 NZ_CP085488:3429225-3429536 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT220804 NZ_CP117293:805615-805881 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3