Detailed information of TA system TA220792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1312908..1313533
• Replicon: chromosome
• Accession: NZ_CP085488
• Organism: Escherichia coli strain VCSW8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LJY68_RS06415
• Protein ID: WP_000911330.1
• Coordinates: 1313135..1313533 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: LJY68_RS06410
• Protein ID: WP_000450524.1
• Coordinates: 1312908..1313135 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJY68_RS06385 (1308711)	1308711..1309181	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
LJY68_RS06390 (1309181)	1309181..1309753	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
LJY68_RS06395 (1309899)	1309899..1310777	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LJY68_RS06400 (1310794)	1310794..1311828	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
LJY68_RS06405 (1312041)	1312041..1312754	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
LJY68_RS06410 (1312908)	1312908..1313135	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LJY68_RS06415 (1313135)	1313135..1313533	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
LJY68_RS06420 (1313680)	1313680..1314543	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
LJY68_RS06425 (1314558)	1314558..1316573	+	2016	WP_227534342.1	tRNA cytosine(34) acetyltransferase TmcA	-
LJY68_RS06430 (1316647)	1316647..1317345	+	699	WP_000679823.1	esterase	-
LJY68_RS06435 (1317455)	1317455..1317655	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T220792 WP_000911330.1 NZ_CP085488:1313135-1313533 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T220792 NZ_CP117292:c537391-537080 [Salmonella enterica subsp. enterica serovar Typhi]
GTGCATGTTATCAGCCGAAAACTGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCGCTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATGAAGAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT220792 WP_000450524.1 NZ_CP085488:1312908-1313135 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT220792 NZ_CP117292:c537083-536637 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33