Detailed information of TA system TA220790

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 843931..844585
• Replicon: chromosome
• Accession: NZ_CP085488
• Organism: Escherichia coli strain VCSW8

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1PAM6
• Locus tag: LJY68_RS04125
• Protein ID: WP_000244781.1
• Coordinates: 844178..844585 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: S1PPB1
• Locus tag: LJY68_RS04120
• Protein ID: WP_000354046.1
• Coordinates: 843931..844197 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJY68_RS04095 (839100)	839100..839843	+	744	WP_000951961.1	SDR family oxidoreductase	-
LJY68_RS04100 (839900)	839900..841333	-	1434	WP_001344773.1	6-phospho-beta-glucosidase BglA	-
LJY68_RS04105 (841378)	841378..841689	+	312	WP_001182954.1	N(4)-acetylcytidine aminohydrolase	-
LJY68_RS04110 (841853)	841853..842512	+	660	WP_000250274.1	hemolysin III family protein	-
LJY68_RS04115 (842708)	842708..843688	-	981	WP_000886095.1	tRNA-modifying protein YgfZ	-
LJY68_RS04120 (843931)	843931..844197	+	267	WP_000354046.1	FAD assembly factor SdhE	Antitoxin
LJY68_RS04125 (844178)	844178..844585	+	408	WP_000244781.1	protein YgfX	Toxin
LJY68_RS04130 (844625)	844625..845146	-	522	WP_001055874.1	flavodoxin FldB	-
LJY68_RS04135 (845258)	845258..846154	+	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
LJY68_RS04140 (846179)	846179..846889	+	711	WP_000715208.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
LJY68_RS04145 (846895)	846895..848628	+	1734	WP_000813218.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16061.99 Da
• Isoelectric Point: 11.5202

>T220790 WP_000244781.1 NZ_CP085488:844178-844585 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T220790 NZ_CP117291:4680508-4680819 [Salmonella enterica subsp. enterica serovar Typhi]
GTGCATGTTATCAGCCGAAAACTGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCGCTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATGAAGAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10547.12 Da
• Isoelectric Point: 5.1599

>AT220790 WP_000354046.1 NZ_CP085488:843931-844197 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT220790 NZ_CP117291:4680816-4681262 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: S1PAM6

Antitoxin

• PDB: 6B58
• PDB: 1X6I
• PDB: 1X6J
• PDB: 6C12
• AlphaFold DB: A0A7U9QD57