Detailed information of TA system TA220782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 87896..88647
• Replicon: plasmid p1
• Accession: NZ_CP085479
• Organism: Klebsiella pneumoniae strain Kp1604

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: LI478_RS25560
• Protein ID: WP_048333033.1
• Coordinates: 87896..88378 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: LI478_RS25565
• Protein ID: WP_004902250.1
• Coordinates: 88369..88647 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LI478_RS25540	84295..84942	-	648	WP_014386537.1	EcsC family protein	-
LI478_RS25545	84969..85724	-	756	WP_004902235.1	DUF2971 domain-containing protein	-
LI478_RS25550	85825..86217	-	393	WP_032442757.1	hypothetical protein	-
LI478_RS25555	86322..86861	-	540	WP_004902239.1	hypothetical protein	-
LI478_RS25560	87896..88378	-	483	WP_048333033.1	GNAT family N-acetyltransferase	Toxin
LI478_RS25565	88369..88647	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
LI478_RS25570	88769..88981	-	213	WP_016946198.1	hypothetical protein	-
LI478_RS25575	89089..89430	-	342	WP_032437747.1	hypothetical protein	-
LI478_RS25580	90336..90650	+	315	WP_023288299.1	hypothetical protein	-
LI478_RS25585	90894..91376	-	483	WP_172694439.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB	1..144892	144892

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17708.53 Da
• Isoelectric Point: 8.9130

>T220782 WP_048333033.1 NZ_CP085479:c88378-87896 [Klebsiella pneumoniae]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T220782 NZ_CP117291:c976422-975895 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACAAAAACCTACGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT220782 WP_004902250.1 NZ_CP085479:c88647-88369 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT220782 NZ_CP117291:c976709-976419 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGATTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3