Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 63208..63944 Replicon plasmid p1
Accession NZ_CP085479
Organism Klebsiella pneumoniae strain Kp1604

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag LI478_RS25435 Protein ID WP_004098919.1
Coordinates 63462..63944 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag LI478_RS25430 Protein ID WP_004213599.1
Coordinates 63208..63474 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI478_RS25405 58440..58997 + 558 WP_004213590.1 recombinase family protein -
LI478_RS25410 59000..61969 + 2970 WP_004213592.1 Tn3 family transposase -
LI478_RS25415 62011..62505 + 495 WP_004213594.1 hypothetical protein -
LI478_RS25420 62566..62769 + 204 WP_004213596.1 HHA domain-containing protein -
LI478_RS25425 62783..63013 + 231 WP_004213598.1 hypothetical protein -
LI478_RS25430 63208..63474 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
LI478_RS25435 63462..63944 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
LI478_RS25440 64365..65592 + 1228 Protein_64 IS3 family transposase -
LI478_RS25445 65588..65983 - 396 Protein_65 IS3 family transposase -
LI478_RS25450 66158..67180 - 1023 WP_004214536.1 porphobilinogen synthase -
LI478_RS25455 67244..68590 - 1347 WP_048333011.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB 1..144892 144892
- inside IScluster/Tn - - 58440..65592 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T220781 WP_004098919.1 NZ_CP085479:63462-63944 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T220781 NZ_CP117291:822597-822995 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCTGAAATTCATGCTTGATACCAATACTTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCACCGG
AGCGTAATTTTGCAGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTACCGAACTGGCCCGCAAGGGAACACCTGTCGGGTCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT220781 WP_004213599.1 NZ_CP085479:63208-63474 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT220781 NZ_CP117291:822370-822597 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGCACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References