Detailed information of TA system TA022078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 852832..853360
• Replicon: chromosome
• Accession: NC_009456
• Organism: Vibrio cholerae O395

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: VC0395_RS03960
• Protein ID: WP_000221352.1
• Coordinates: 853070..853360 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H9L4T4
• Locus tag: VC0395_RS03955
• Protein ID: WP_000213183.1
• Coordinates: 852832..853080 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VC0395_RS03910	847872..848336	+	465	WP_000140244.1	hypothetical protein	-
VC0395_RS03915	848436..848834	-	399	WP_001882293.1	hypothetical protein	-
VC0395_RS03920	848990..849490	-	501	WP_000789723.1	hypothetical protein	-
VC0395_RS03925	849628..850158	-	531	WP_000415562.1	hypothetical protein	-
VC0395_RS03930	850309..850704	-	396	WP_000649527.1	DUF3465 domain-containing protein	-
VC0395_RS03935	850912..851337	-	426	WP_000415748.1	hypothetical protein	-
VC0395_RS03940	851608..852261	-	654	WP_000036957.1	site-specific DNA-methyltransferase	-
VC0395_RS03955	852832..853080	+	249	WP_000213183.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
VC0395_RS03960	853070..853360	+	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VC0395_RS03965	853496..854011	-	516	WP_001201531.1	lipocalin family protein	-
VC0395_RS03970	854148..854624	-	477	WP_001047171.1	GNAT family N-acetyltransferase	-
VC0395_RS20550	854652..854762	-	111	WP_071917832.1	DUF3265 domain-containing protein	-
VC0395_RS03975	854759..855688	-	930	WP_000335802.1	hypothetical protein	-
VC0395_RS03980	855808..856254	-	447	WP_000128977.1	hypothetical protein	-
VC0395_RS03985	856406..857410	-	1005	WP_000964934.1	LD-carboxypeptidase	-
VC0395_RS21060	857623..857661	-	39	WP_106019119.1	hypothetical protein	-
VC0395_RS21065	857677..857802	-	126	WP_001944767.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	833458..916350	82892

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T22078 WP_000221352.1 NC_009456:853070-853360 [Vibrio cholerae O395]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T22078 NC_009456:853070-853360 [Vibrio cholerae O395]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8937.28 Da
• Isoelectric Point: 4.1079

>AT22078 WP_000213183.1 NC_009456:852832-853080 [Vibrio cholerae O395]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAIAKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT22078 NC_009456:852832-853080 [Vibrio cholerae O395]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A366A3W8