Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5127261..5127886 Replicon chromosome
Accession NZ_CP085478
Organism Klebsiella pneumoniae strain Kp1604

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LI478_RS24760 Protein ID WP_002882817.1
Coordinates 5127261..5127644 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LI478_RS24765 Protein ID WP_004150355.1
Coordinates 5127644..5127886 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI478_RS24745 5124627..5125529 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LI478_RS24750 5125526..5126161 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LI478_RS24755 5126158..5127087 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LI478_RS24760 5127261..5127644 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LI478_RS24765 5127644..5127886 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LI478_RS24770 5128091..5129008 + 918 WP_012737090.1 alpha/beta hydrolase -
LI478_RS24775 5129022..5129963 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
LI478_RS24780 5130008..5130445 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LI478_RS24785 5130442..5131302 - 861 WP_004146232.1 virulence factor BrkB family protein -
LI478_RS24790 5131296..5131895 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T220779 WP_002882817.1 NZ_CP085478:c5127644-5127261 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T220779 NZ_CP117291:205084-205569 [Salmonella enterica subsp. enterica serovar Typhi]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGGGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGACGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT220779 WP_004150355.1 NZ_CP085478:c5127886-5127644 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT220779 NZ_CP117291:204809-205096 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAGTCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCGGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References