Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4536326..4537136 Replicon chromosome
Accession NZ_CP085478
Organism Klebsiella pneumoniae strain Kp1604

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LI478_RS21955 Protein ID WP_004178461.1
Coordinates 4536326..4536859 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LI478_RS21960 Protein ID WP_002887278.1
Coordinates 4536870..4537136 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI478_RS21950 4535157..4536278 + 1122 WP_064153030.1 cupin domain-containing protein -
LI478_RS21955 4536326..4536859 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LI478_RS21960 4536870..4537136 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LI478_RS21965 4537239..4538672 - 1434 WP_009484674.1 Cu(+)/Ag(+) sensor histidine kinase -
LI478_RS21970 4538662..4539345 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
LI478_RS21975 4539518..4540903 + 1386 WP_064153031.1 efflux transporter outer membrane subunit -
LI478_RS21980 4540921..4541265 + 345 WP_048266266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T220776 WP_004178461.1 NZ_CP085478:c4536859-4536326 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T220776 NZ_CP117290:c4246167-4245676 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGTGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT220776 WP_002887278.1 NZ_CP085478:c4537136-4536870 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT220776 NZ_CP117290:c4246457-4246164 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGTGAAACTCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGTTGGAAGCTGCCCGTGCTGCCGCAGAAGAAGTGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAATTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References