Detailed information of TA system TA220774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2579373..2580961
• Replicon: chromosome
• Accession: NZ_CP085478
• Organism: Klebsiella pneumoniae strain Kp1604

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486JB51
• Locus tag: LI478_RS12620
• Protein ID: WP_023316847.1
• Coordinates: 2579373..2580695 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LI478_RS12625
• Protein ID: WP_004176198.1
• Coordinates: 2580695..2580961 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LI478_RS12605	2574429..2576930	+	2502	WP_064153112.1	fimbria/pilus outer membrane usher protein	-
LI478_RS12610	2576918..2577922	+	1005	WP_004176196.1	fimbrial protein	-
LI478_RS12615	2578064..2579356	+	1293	WP_032414911.1	glycoside hydrolase family 10 protein	-
LI478_RS12620	2579373..2580695	-	1323	WP_023316847.1	type II toxin-antitoxin system HipA family toxin	Toxin
LI478_RS12625	2580695..2580961	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LI478_RS12630	2581348..2581674	-	327	WP_004224757.1	hydroxyisourate hydrolase	-
LI478_RS12635	2581671..2582171	-	501	WP_004224756.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LI478_RS12640	2582149..2583528	-	1380	WP_004184021.1	nucleobase:cation symporter-2 family protein	-
LI478_RS12645	2583874..2585028	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LI478_RS12650	2585009..2585932	-	924	WP_004184020.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49141.44 Da
• Isoelectric Point: 7.5357

>T220774 WP_023316847.1 NZ_CP085478:c2580695-2579373 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T220774 NZ_CP117290:c4006438-4006154 [Salmonella enterica subsp. enterica serovar Typhi]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT220774 WP_004176198.1 NZ_CP085478:c2580961-2580695 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT220774 NZ_CP117290:c4006670-4006428 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A486JB51