Detailed information of TA system TA220765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1062569..1063308
• Replicon: chromosome
• Accession: NZ_CP085478
• Organism: Klebsiella pneumoniae strain Kp1604

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: LI478_RS05270
• Protein ID: WP_021312536.1
• Coordinates: 1062569..1063054 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LI478_RS05275
• Protein ID: WP_003026799.1
• Coordinates: 1063042..1063308 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LI478_RS05245	1057697..1059052	+	1356	WP_032415797.1	aromatic acid/H+ symport family MFS transporter	-
LI478_RS05250	1059298..1059588	+	291	WP_002921931.1	HTH-type transcriptional regulator	-
LI478_RS05255	1059841..1060053	+	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
LI478_RS05260	1060152..1061771	-	1620	WP_032415799.1	ABC-F family ATP-binding cassette domain-containing protein	-
LI478_RS05265	1062073..1062225	-	153	WP_002922102.1	type I toxin-antitoxin system toxin HokA	-
LI478_RS05270	1062569..1063054	-	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
LI478_RS05275	1063042..1063308	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LI478_RS05280	1063441..1063869	-	429	WP_004901287.1	GFA family protein	-
LI478_RS05290	1065736..1067805	-	2070	WP_002922127.1	glycine--tRNA ligase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1064070..1064702	632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T220765 WP_021312536.1 NZ_CP085478:c1063054-1062569 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T220765 NZ_CP117289:c923101-922256 [Streptococcus dysgalactiae subsp. equisimilis]
ATGAACAAAGCTAAATTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGTTTAACCTTTAATTCCGTGATGACCTA
TTACTTCCTAGAAGTCATTTTGAAAAAATTAAGTCAAAGTTCTTATTCAAATCACTATATCTTCAAAGGAGGATTCCTAC
TGTCGAATGTCATCGGAGTTGAATCTCGTAGCACTGTTGATATTGACTTTTTATTCCATCAAATAACACTCTCAGAAGAA
ACTGTTAAACAGCAACTCAAGGAAATTTTAGCAGATTCCGAAGAAGGTATATCTTTTGTGATTCAATCAATCACAACTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGCAACAATTTCGTGCCAACTTGAGAATATTAAACAAGTTATCCATTTGG
ATATTGCGACAGGTGATGTTGTAACTCCTCAGCCGATAACATACGACTATAAAGCTATTTTTGATGAAGACAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAAAAACTCCAAACCATCTATTCACGAAACTTCTTGAACAGCAGAAG
CAAAGACTTTTACGATGTTTATATTCTTTCAAAATTGAAGAAAAAAGATATTGATTTCAATCAGTTAAAAAATGCTTGCC
AAAGAACTTTTTCCTATCGCGAAACTGAGCTCGATTTCGAAAAAATTATTGAACTACTCGAGAGATTCAAATCTGATCCT
ACTCAGAATCAACAATGGCAAAATTATTCAAAAAAATATAGTTATACAAAAGGAATTTCACTTGCAAATGTGCTTGATGA
GATGATTAGTCTTATTACAATTATTATTTCTACTGACTCTGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT220765 WP_003026799.1 NZ_CP085478:c1063308-1063042 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT220765 NZ_CP117289:c923688-923098 [Streptococcus dysgalactiae subsp. equisimilis]
ATGTCAAAAAAAGAGATTCTACTCGATTTTATAGAAAAGAACAATGGCATTGTCACAAACAAAGATTGTAAAGCACTAGG
TATTCCAACAATATATCTGACAAGACTAGAGAAAGAAGGCATTATCTTCCGAGTTGAAAAAGGTATTTTCCTAACCCAAA
ACGGAGACTATGATGAATACTACTTCTTTCAATATCGTTTTCCTAAGGCTATTTTCTCTTATATTTCGGCACTGTATCTA
CAACAGTTTACAGATGAAATTCCACAATATTTTGATGTGACAGTTCCTAGAGGCTACCGCTTTAATACTCCTCCAGCTAA
TCTAAACATTCACTTTGTCTCTAAAGAATACAGCGAGCTGGGAATGACTACTGTACCTACTCCTATGGGAAACAACGTAA
GGGTATATGATTTTGAACGTATTATTTGTGATTTTGTAATACATCGAGAAAAGATTGATTCCGAACTCTTTGTCAAGACA
CTTCAATCTTATGGGAACTATCCTAAAAAAAATCTAGCAAAACTCTATGAATATGCGACAAAAATGAATACTTTAGAAAA
AGTTAAACAAACTCTGGAGGTTCTAATATGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66