Detailed information of TA system TA220761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-pasA/RelE-HTH
• Location: 2227..2698
• Replicon: plasmid p71485-1
• Accession: NZ_CP085469
• Organism: Aeromonas caviae strain 71485

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LEO80_RS21590
• Protein ID: WP_227263754.1
• Coordinates: 2435..2698 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: LEO80_RS21585
• Protein ID: WP_227263753.1
• Coordinates: 2227..2451 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LEO80_RS21570 (LEO80_21570)	216..629	+	414	WP_227263752.1	hypothetical protein	-
LEO80_RS21575 (LEO80_21575)	948..1220	-	273	WP_139743096.1	hypothetical protein	-
LEO80_RS21580 (LEO80_21580)	1241..1624	-	384	WP_139743097.1	hypothetical protein	-
LEO80_RS21585 (LEO80_21585)	2227..2451	+	225	WP_227263753.1	DUF6290 family protein	Antitoxin
LEO80_RS21590 (LEO80_21590)	2435..2698	+	264	WP_227263754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LEO80_RS21595 (LEO80_21595)	2879..3817	+	939	WP_227263755.1	replication initiation protein	-
LEO80_RS21600 (LEO80_21600)	4489..4890	+	402	WP_227263749.1	plasmid mobilization relaxosome protein MobC	-
LEO80_RS21605 (LEO80_21605)	4883..5569	+	687	Protein_7	relaxase/mobilization nuclease domain-containing protein	-
LEO80_RS21610 (LEO80_21610)	5595..6257	+	663	WP_227263750.1	hypothetical protein	-
LEO80_RS21615 (LEO80_21615)	6258..6620	+	363	WP_227263751.1	mobilization protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..6698	6698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• Relaxosome protein TraY (4-47)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10170.81 Da
• Isoelectric Point: 9.7744

>T220761 WP_227263754.1 NZ_CP085469:2435-2698 [Aeromonas caviae]
MAWKVELTDTAVKQLARLDKTQAQRITKYLRRVMMLEDPRDAGKALTGNLRTYWRYRVGDYRVICEIRDAELVIVAVEIG
HRSEVYG

Nucleotide

• Length: 264 bp

>T220761 NZ_CP117288:c289041-288787 [Streptococcus dysgalactiae subsp. equisimilis]
ATGTTTAATTTTACTGAAGAGGCTTGGGAAGATTACACTTCTTGGCAAAGAGAAGATAAAAAAAATCTCAAGCGTATTAA
TCGACTGATTGAAGATATCAAACGTCACCCTTTCGAAGGAATCGGAAAACCAGAACCTTTAAAATATCGGTATTCTGGTG
CCTGGTCTCGCCGTATTACAGACGAACACCGTCTCGTTTATACGGTAGAACAAAATGATATCTATTTCTTATCTTTTAGA
GATCACTATAAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8725.85 Da
• Isoelectric Point: 4.4755

>AT220761 WP_227263753.1 NZ_CP085469:2227-2451 [Aeromonas caviae]
MLAIRLPSEIEERLAHLAQETGRTKTYYAREAILTHLEDLEDYYLAERVMEKVRKGEERLFSLDDVERDLGLES

Nucleotide

• Length: 225 bp

>AT220761 NZ_CP117288:c289300-289034 [Streptococcus dysgalactiae subsp. equisimilis]
ATGGAAGCTGTTGTTTATTCAAATTTTAGAAACAATTTAAAAGATTACATGAAAAAGGTAAATGATGAATACGAGCCATT
AATGGTAGTTAATAAAAACCCTGAAGAAAATATCGTGGTCTTGTCTAAAGACAATTGGGATAGTATTCAAGAAACCATTC
GTATCATGAATAACGAATACCTCTCTGATAAAGTTCTATCAGGATTGGAACAAGTCAAACAAAAGAAAGTGACCCAACAC
CAATTATTAGAGGTGGAAGATGTTTAA