Detailed information of TA system TA220757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 200580..201216
• Replicon: chromosome
• Accession: NZ_CP085442
• Organism: Priestia megaterium strain KF18

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: LIS78_RS01140
• Protein ID: WP_013055004.1
• Coordinates: 200866..201216 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: LIS78_RS01135
• Protein ID: WP_013055003.1
• Coordinates: 200580..200861 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIS78_RS01110 (LIS78_01110)	195946..196917	+	972	WP_252284543.1	UV DNA damage repair endonuclease UvsE	-
LIS78_RS01115 (LIS78_01115)	196926..197528	-	603	WP_098250182.1	rhomboid family intramembrane serine protease	-
LIS78_RS01120 (LIS78_01120)	197593..197958	+	366	WP_195783335.1	holo-ACP synthase	-
LIS78_RS01125 (LIS78_01125)	198017..199075	+	1059	WP_195781428.1	outer membrane lipoprotein carrier protein LolA	-
LIS78_RS01130 (LIS78_01130)	199189..200379	+	1191	WP_195781427.1	alanine racemase	-
LIS78_RS01135 (LIS78_01135)	200580..200861	+	282	WP_013055003.1	hypothetical protein	Antitoxin
LIS78_RS01140 (LIS78_01140)	200866..201216	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
LIS78_RS01145 (LIS78_01145)	201376..202212	+	837	WP_252284544.1	RsbT co-antagonist protein RsbRA	-
LIS78_RS01150 (LIS78_01150)	202215..202571	+	357	WP_013055006.1	STAS domain-containing protein	-
LIS78_RS01155 (LIS78_01155)	202575..202976	+	402	WP_013055007.1	anti-sigma regulatory factor	-
LIS78_RS01160 (LIS78_01160)	202990..204000	+	1011	WP_195781426.1	PP2C family protein-serine/threonine phosphatase	-
LIS78_RS01165 (LIS78_01165)	204060..204392	+	333	WP_013055009.1	anti-sigma factor antagonist	-
LIS78_RS01170 (LIS78_01170)	204389..204874	+	486	WP_025753516.1	anti-sigma B factor RsbW	-
LIS78_RS01175 (LIS78_01175)	204840..205634	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T220757 WP_013055004.1 NZ_CP085442:200866-201216 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T220757 NZ_CP117285:c2158569-2157802 [Streptococcus dysgalactiae subsp. equisimilis]
ATGAAACTAGAAGAATTTAGTCAAGATAATTTTGAACAAGCCTCCAAACGACTCGTTCGTTCTTTAACTCGTGGAAAAAC
AACCTCATCTCAGCCTAAGGCTATTTTGCTCGGTGGACAGAGTGGTGCTGGGAAAACAACGATCCATCGAATTAAACAAA
GGGAATTTCAAGGTAATATCATTATTATTGATGGAGATAGTTATCGCTCTTTTCATCCGAACTACCTTGGCCTACAGGAA
AGGTATGGCAAAGATAGTGTGGATTATACCAAAGTATTCGCGGGACAAATGGTTGAATATCTTGTAGATGAGTTGAGTAA
AAAAGGTTATCATTTATTAATCGAGGGTACCTTGAGAACAACAGAAGTTCCCAAAAAAACTGCACAATTATTGACAACTA
AAGGTTACCAAGTCTCACTTGCAGTAATTGCGACCAAGCCAGAATTGTCCTATCTCAGTACCTTGATACGTTATGAAGAG
CTCCATGCTATAGACCCTAGTCAAGCTAGAGCGACTCCCAAAGAGCACCATGATGGAATTGTAGAACATTTAGTTGATAA
CTTACGAGAGTTAGAAAATGACAAAATTTTTAATCAAATTCAAATTTATCAAAGAGATCAATCGTGTATTTATGATTCGC
AAGTAGATCAAATCTCAGCAGCAGAAGTTTTACAAGAATGCCTCTTCGGAAAATGGAGTAAGGTGGAGGAAGAGATGTTG
AAGTTGGGACAGGAACGGTTGAAAGAGCTGGGGGAAATTGATAAGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT220757 WP_013055003.1 NZ_CP085442:200580-200861 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT220757 NZ_CP117285:c2159045-2158569 [Streptococcus dysgalactiae subsp. equisimilis]
ATGATTGGAGAAAATATTAAAACGTTACGCAAGGCACATAATCTAACTCAGCCTGAGTTCGCAAAGATTGTTGGTATCTC
TCGAAATAGTCTGAGTCGTTATGAAAATGGGACAAGTCGAGTTTCGACAGAATTAATAGATCGTATCTGCAAGAAATTTA
ATGTTTCTTATATCGATATCGTAGGGGAGGAAAAAATGCTCACACCAGTAGAAGATTATCAGTTAACTCTGAAAATTGAA
GTGATTAAAGAACGAGGGGCGAATATCTTAGCGCAACTTTATCGTTTTCAAGATGAACAAGGAATTGCATTTGATGATAC
TTCTAATCCTTGGGTTCTAATGAGCGATGATTTGTCTGATTTAATTAACACGAAGATTTATCTTGTTGCAACTTTTGAGG
ATATAGAGCGATTTAATGGCTATTTGGATGGCATTGAACGTATGCTAGAACAAGCTACTCATCTGGTGGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9