Detailed information of TA system TA220756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 200621..201257
• Replicon: chromosome
• Accession: NZ_CP085431
• Organism: Priestia megaterium strain F20

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: LIT34_RS01135
• Protein ID: WP_013055004.1
• Coordinates: 200907..201257 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: LIT34_RS01130
• Protein ID: WP_013055003.1
• Coordinates: 200621..200902 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT34_RS01105 (LIT34_01105)	195987..196958	+	972	WP_035439664.1	UV DNA damage repair endonuclease UvsE	-
LIT34_RS01110 (LIT34_01110)	196967..197569	-	603	WP_013054999.1	rhomboid family intramembrane serine protease	-
LIT34_RS01115 (LIT34_01115)	197634..197999	+	366	WP_013055000.1	holo-ACP synthase	-
LIT34_RS01120 (LIT34_01120)	198058..199116	+	1059	WP_013055001.1	outer membrane lipoprotein carrier protein LolA	-
LIT34_RS01125 (LIT34_01125)	199230..200420	+	1191	WP_013055002.1	alanine racemase	-
LIT34_RS01130 (LIT34_01130)	200621..200902	+	282	WP_013055003.1	hypothetical protein	Antitoxin
LIT34_RS01135 (LIT34_01135)	200907..201257	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
LIT34_RS01140 (LIT34_01140)	201415..202251	+	837	WP_013055005.1	RsbT co-antagonist protein RsbRA	-
LIT34_RS01145 (LIT34_01145)	202254..202610	+	357	WP_013055006.1	STAS domain-containing protein	-
LIT34_RS01150 (LIT34_01150)	202614..203015	+	402	WP_013055007.1	anti-sigma regulatory factor	-
LIT34_RS01155 (LIT34_01155)	203029..204039	+	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
LIT34_RS01160 (LIT34_01160)	204099..204431	+	333	WP_013055009.1	anti-sigma factor antagonist	-
LIT34_RS01165 (LIT34_01165)	204428..204913	+	486	WP_013055010.1	anti-sigma B factor RsbW	-
LIT34_RS01170 (LIT34_01170)	204879..205673	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T220756 WP_013055004.1 NZ_CP085431:200907-201257 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T220756 NZ_CP117285:c892398-891553 [Streptococcus dysgalactiae subsp. equisimilis]
ATGAACAAAGCTAAATTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGTTTAACCTTTAATTCCGTGATGACCTA
TTACTTCCTAGAAGTCATTTTGAAAAAATTAAGTCAAAGTTCTTATTCAAATCACTATATCTTCAAAGGAGGATTCCTAC
TGTCGAATGTCATCGGAGTTGAATCTCGTAGCACTGTTGATATTGACTTTTTATTCCATCAAATAACACTCTCAGAAGAA
ACTGTTAAACAGCAACTCAAGGAAATTTTAGCAGATTCCGAAGAAGGTATATCTTTTGTGATTCAATCAATCACAACTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGCAACAATTTCGTGCCAACTTGAGAATATTAAACAAGTTATCCATTTGG
ATATTGCGACAGGTGATGTTGTAACTCCTCAGCCGATAACATACGACTATAAAGCTATTTTTGATGAAGACAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAAAAACTCCAAACCATCTATTCACGAAACTTCTTGAACAGCAGAAG
CAAAGACTTTTACGATGTTTATATTCTTTCAAAATTGAAGAAAAAAGATATTGATTTCAATCAGTTAAAAAATGCTTGCC
AAAGAACTTTTTCCTATCGCGAAACTGAGCTCGATTTCGAAAAAATTATTGAACTACTCGAGAGATTCAAATCTGATCCT
ACTCAGAATCAACAATGGCAAAATTATTCAAAAAAATATAGTTATACAAAAGGAATTTCACTTGCAAATGTGCTTGATGA
GATGATTAGTCTTATTACAATTATTATTTCTACTGACTCTGATTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT220756 WP_013055003.1 NZ_CP085431:200621-200902 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT220756 NZ_CP117285:c892985-892395 [Streptococcus dysgalactiae subsp. equisimilis]
ATGTCAAAAAAAGAGATTCTACTCGATTTTATAGAAAAGAACAATGGCATTGTCACAAACAAAGATTGTAAAGCACTAGG
TATTCCAACAATATATCTGACAAGACTAGAGAAAGAAGGCATTATCTTCCGAGTTGAAAAAGGTATTTTCCTAACCCAAA
ACGGAGACTATGATGAATACTACTTCTTTCAATATCGTTTTCCTAAGGCTATTTTCTCTTATATTTCGGCACTGTATCTA
CAACAGTTTACAGATGAAATTCCACAATATTTTGATGTGACAGTTCCTAGAGGCTACCGCTTTAATACTCCTCCAGCTAA
TCTAAACATTCACTTTGTCTCTAAAGAATACAGCGAGCTGGGAATGACTACTGTACCTACTCCTATGGGAAACAACGTAA
GGGTATATGATTTTGAACGTATTATTTGTGATTTTGTAATACATCGAGAAAAGATTGATTCCGAACTCTTTGTCAAGACA
CTTCAATCTTATGGGAACTATCCTAAAAAAAATCTAGCAAAACTCTATGAATATGCGACAAAAATGAATACTTTAGAAAA
AGTTAAACAAACTCTGGAGGTTCTAATATGA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9